Joly J R, Boissinot M, Duchaine J, Duval M, Rafrafi J, Ramsay D, Letarte R
Can J Microbiol. 1984 Jan;30(1):63-7. doi: 10.1139/m84-011.
One hundred environmental water samples, which were collected in the Quebec city area and cultured on buffered charcoal yeast extract medium and three selective media, were inoculated to guinea pigs and were screened by direct immunofluorescent staining (DFA) for the presence of Legionellaceae. Six isolates were made (four Legionella pneumophila and two Tatlockia (Legionella) micdadei: three by animal inoculation and three by culture). No samples were simultaneously positive by both methods. After screening by DFA, 43 of the 100 samples were positive for Legionellaceae and 27 of those contained more than one serogroup and (or) species of Legionellaceae. Legionella pneumophila (serogroups 1 to 6) was the most frequent species seen by DFA. These results clearly show that Legionellaceae are frequent members of the freshwater microbial flora of the Quebec city area.
在魁北克市地区采集了100份环境水样，将其接种于缓冲活性炭酵母提取物培养基和三种选择性培养基上进行培养，然后接种到豚鼠体内，并通过直接免疫荧光染色（DFA）筛查是否存在军团菌科细菌。共分离出6株菌株（4株嗜肺军团菌和2株米克戴德军团菌（塔特洛克军团菌）：3株通过动物接种分离得到，3株通过培养分离得到）。两种方法均未同时检测出阳性样本。经DFA筛查，100份样本中有43份军团菌科细菌呈阳性，其中27份含有不止一个血清群和（或）军团菌科细菌物种。嗜肺军团菌（血清群1至6）是DFA检测中最常见的物种。这些结果清楚地表明，军团菌科细菌是魁北克市地区淡水微生物群落的常见成员。
