在添加白蛋白的缓冲活性炭酵母提取物琼脂上，米克戴德军团菌和博兹曼军团菌的回收率增加。
Increased recovery of Legionella micdadei and Legionella bozemanii on buffered charcoal yeast extract agar supplemented with albumin.
作者信息
Morrill W E, Barbaree J M, Fields B S, Sanden G N, Martin W T
机构信息
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1990 Mar;28(3):616-8. doi: 10.1128/jcm.28.3.616-618.1990.
The recovery of Legionella micdadei and L. bozemanii serogroups 1 and 2 from infected guinea pig spleens was evaluated by using two culture media: buffered charcoal yeast extract agar with 0.1% alpha-ketoglutarate (BCYE alpha) and the same medium supplemented with 1% bovine serum albumin (ABCYE alpha). At the lowest dilution of spleen tissue (10(-1)), recovery of all strains of L. micdadei and L. bozemanii was more efficient on ABCYE alpha than on BCYE alpha. L. micdadei strains had higher recovery rates on ABCYE alpha after another 10-fold dilution, but recoveries of L. bozemanii were similar on both media. Recovery rates for most test strains were comparable on BCYE alpha and ABCYE alpha at the highest dilution (10(-3)) of tissue tested. The presence of albumin in BCYE alpha increased the recovery rate of L. micdadei more than that of L. bozemanii. The use of ABCYE alpha medium in place of BCYE alpha may improve the recovery of L. micdadei and L. bozemanii from clinical specimens. Preliminary studies indicate that this medium also enhances recovery of certain Legionella spp. from environmental samples.
通过使用两种培养基，即添加0.1%α-酮戊二酸的缓冲活性炭酵母提取物琼脂（BCYEα）和添加1%牛血清白蛋白的相同培养基（ABCYEα），评估了从感染豚鼠脾脏中分离米克戴德军团菌及博兹曼军团菌血清群1和血清群2的情况。在脾脏组织最低稀释度（10⁻¹）时，所有米克戴德军团菌和博兹曼军团菌菌株在ABCYEα上的回收率均高于在BCYEα上的回收率。在再进行10倍稀释后，米克戴德军团菌菌株在ABCYEα上的回收率更高，但博兹曼军团菌在两种培养基上的回收率相似。在测试组织的最高稀释度（10⁻³）时，大多数测试菌株在BCYEα和ABCYEα上的回收率相当。BCYEα中白蛋白的存在对米克戴德军团菌回收率的提高作用大于对博兹曼军团菌的作用。用ABCYEα培养基替代BCYEα可能会提高从临床标本中分离米克戴德军团菌和博兹曼军团菌的回收率。初步研究表明，这种培养基还能提高从环境样本中分离某些军团菌属的回收率。
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