Yulis C R, Peruzzo B, Rodríguez E M
Cell Tissue Res. 1984;236(1):171-80. doi: 10.1007/BF00216528.
The neuropil located ventral to the SON was investigated by the use of immunoperoxidase staining for neurophysins, oxytocin and vasopressin, and electron microscopy. The study was performed in six groups of rats: 1) control; 2) infusion of isotonic saline into the CSF; 3) infusion of hypertonic saline into the CSF; 4) drinking hypertonic saline for 4 days; 5) same as group 4 but injection of colchicine into the CSF on second day of dehydration; 6) salt loading for 3 months. In the control rats the ventral neuropil contained a few immunoreactive processes, the general morphology of which was completely different from that of the neurosecretory axons emerging from the SON at its dorsal aspect. In rats of groups 3 to 6 the ventral processes (VP) became loaded with neurosecretory granules, whereas the perikarya and axons were depleted. Based on their general morphology and reactivity pattern it is suggested that the VP are dendrites. Most of these "dendrites" were embedded in a glial cushion formed by the processes of a particular type of marginal glia. Some of these "dendrites" enveloped an arteriole penetrating the optic tract. All VP were rich in synaptic contacts. The possibility that the VP of neurosecretory cells may be functionally related to the subarachnoid CSF and the arteriolar blood flow is discussed.
运用神经垂体激素、催产素和加压素的免疫过氧化物酶染色法以及电子显微镜技术，对位于视上核腹侧的神经纤维网进行了研究。该研究在六组大鼠中开展：1）对照组；2）向脑脊液中注入等渗盐水；3）向脑脊液中注入高渗盐水；4）饮用高渗盐水4天；5）与第4组相同，但在脱水第二天向脑脊液中注射秋水仙碱；6）高盐负荷3个月。在对照大鼠中，腹侧神经纤维网含有一些免疫反应阳性的突起，其总体形态与从视上核背侧发出的神经分泌轴突完全不同。在第3至6组大鼠中，腹侧突起（VP）充满了神经分泌颗粒，而胞体和轴突则减少。根据它们的总体形态和反应模式，推测VP是树突。这些“树突”大多嵌入由一种特殊类型的边缘神经胶质细胞的突起形成的神经胶质垫中。其中一些“树突”包裹着一条穿透视束的小动脉。所有VP都富含突触联系。文中讨论了神经分泌细胞的VP可能在功能上与蛛网膜下腔脑脊液和小动脉血流相关的可能性。
