Duggan D E, Hooke K F, Ramjit H G
J Chromatogr. 1984 Mar 9;306:69-77. doi: 10.1016/s0378-4347(00)80870-1.
A simple and specific gas-liquid chromatographic procedure, compatible with both nitrogen-phosphorous and electron-capture detection, and employing conventional packed columns, has been devised for urinary 1-methylimidazole-4-acetic acid (N tau-MeImAA), an index of whole-body histamine turnover. N tau-MeImAA is isolated by ion-exchange on Dowex 1 (CH3COO-), esterified by reaction with chloroethanol-boron trichloride and, depending upon detection employed, chromatographed on base-deactivated SP-2401 or SP-2250. [3H]N tau-MeImAA serves as internal recovery standard.
已设计出一种简单且特异的气液色谱法，该方法与氮磷检测和电子捕获检测均兼容，且采用常规填充柱，用于检测尿中1-甲基咪唑-4-乙酸（Nτ-甲基咪唑乙酸，N tau-MeImAA），它是全身组胺代谢的一个指标。Nτ-甲基咪唑乙酸通过在Dowex 1（CH3COO-）上进行离子交换分离，与氯乙醇-三氯化硼反应进行酯化，然后根据所采用的检测方法，在碱钝化的SP-2401或SP-2250上进行色谱分析。[3H]Nτ-甲基咪唑乙酸用作内部回收率标准。
