Chikazawa S, Nunoi H, Endo F, Matsuda I, Honda M
Pediatr Res. 1984 Apr;18(4):365-9. doi: 10.1203/00006450-198404000-00012.
An 8-yr-old girl with a history of severe recurrent infections including perinephritic, pulmonary, and hepatic abscesses had elevated serum IgE levels. Her serum inhibited chemotaxis of polymorphonuclear leukocytes (PMN) and monocytes. Exchange blood transfusion or plasma exchange at the time of severe infection resulted in normalization of chemotactic activity of PMN shown by the skin window method. Although this effect became negative 1 wk after the treatment, the procedures improved her clinical course. The patient's serum, obtained by exchange blood transfusion, 1) inhibited normal PMN chemotaxis toward cultured supernatant of E. coli, zymosan-activated serum, and formyl methionyl-leucyl-phenylalanine (f . Met-Leu-Phe), a synthetic chemotactic peptide; 2) inhibited monocyte chemotaxis, 3) showed an absence of digestive activity of f . Met-Leu-Phe, 4) was heat stable at 56 degrees C for 30 min and 5) showed an absence of antigenicity of IgE in a partial purified inhibitor with a molecular weight of 30,000-40,000. The inhibitory effect seemed to be reversible.
一名8岁女童有严重反复感染病史，包括肾周、肺部和肝脓肿，其血清IgE水平升高。她的血清抑制多形核白细胞（PMN）和单核细胞的趋化作用。在严重感染时进行换血输血或血浆置换，通过皮肤窗法显示PMN趋化活性恢复正常。尽管治疗1周后这种效果消失，但这些操作改善了她的临床病程。通过换血输血获得的患者血清，1）抑制正常PMN对大肠杆菌培养上清液、酵母聚糖激活血清和甲酰甲硫氨酰亮氨酰苯丙氨酸（f.Met-Leu-Phe）（一种合成趋化肽）的趋化作用；2）抑制单核细胞趋化作用；3）对f.Met-Leu-Phe无消化活性；4）在56℃下30分钟热稳定；5）在分子量为30,000 - 40,000的部分纯化抑制剂中显示无IgE抗原性。这种抑制作用似乎是可逆的。
