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猪水疱病病毒与柯萨奇B5病毒的对比电泳检测

A comparative electrophoretic examination of swine vesicular disease virus and Coxsackie B5 virus.

作者信息

Sorensen K J

出版信息

Arch Virol. 1977;53(3):235-41. doi: 10.1007/BF01314667.

DOI:10.1007/BF01314667
PMID:193464
Abstract

Purified suspensions of Coxsackie B5 virus and swine vesicular disease virus (SVDV) were prepared by harvesting and purifying cell pack virus. Crossed immunoelectrophoresis was carried out with purified N and H antigen fractions (full and empty particles). Relative migration velocity (RMV) was calculated for the N antigen fraction of 3 SVD viruses (UKG72, HK71 and Italy 66) and 2 Coxsackie B5 viruses (Faulkner and 8068). The prototype strain of Coxsackie B5 virus (Faulkner) had a low RMV almost identical to that of the first isolated strain of SVDV, Italy 66. A recent isolate of Coxsackie B5 virus (8068, isolated in UK in 1973) had a relatively high RMV very close to that of the UKG72 strain of SVDV isolated in the UK in 1972. Also the Hong Kong strain of SVDV (HK71) had a high RMV value. These observations are considered in relation to the emergence of swine vesicular disease.

摘要

通过收获和纯化细胞包病毒制备了柯萨奇B5病毒和猪水疱病病毒(SVDV)的纯化悬液。用纯化的N和H抗原组分(完整和空颗粒)进行交叉免疫电泳。计算了3种SVD病毒(UKG72、HK71和意大利66)和2种柯萨奇B5病毒(福克纳和8068)的N抗原组分的相对迁移速度(RMV)。柯萨奇B5病毒的原型株(福克纳)的RMV较低,几乎与首次分离的SVDV株意大利66相同。最近分离的一株柯萨奇B5病毒(8068,1973年在英国分离)的RMV相对较高,与1972年在英国分离的SVDV的UKG72株非常接近。此外,SVDV的香港株(HK71)的RMV值也很高。结合猪水疱病的出现对这些观察结果进行了讨论。

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引用本文的文献

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2
A comparative investigation of antibody to swine vesicular disease virus using counter immunoelectrophoresis, serum neutralization and double immunodiffusion tests.运用对流免疫电泳、血清中和试验及双向免疫扩散试验对猪水疱病病毒抗体进行的对比研究。
Acta Vet Scand. 1980;21(3):318-23. doi: 10.1186/BF03546862.

本文引用的文献

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