Noble S A, Fisher E F, Caruthers M H
Nucleic Acids Res. 1984 Apr 11;12(7):3387-404. doi: 10.1093/nar/12.7.3387.
Deoxydinucleoside methylphosphonates were prepared by chemical synthesis and were introduced stereospecifically into the lac operator at two sites. These sites within d(ApApTpTpGpTpGpApGpCpGpGpApTpApApCpApApTpT), segment I, and d(ApApTpTpGpTpTpApTpCpCpGpCpTpCpApCpApApTpT), segment II, are indicated by p. Each segment containing a chiral methylphosphonate was annealed to the complementary unmodified segment. The interactions of these four modified lac operators with lac repressor were analyzed by the nitrocellulose filter binding assay. Introduction of either chiral phosphonate in segment II had little effect on the stability of the repressor-operator complex. When methylphosphonates were introduced into segment I, the affinity of lac repressor for the modified operators was shown to be dependent on the stereochemical configuration of the methylphosphonate.
脱氧二核苷甲基膦酸酯通过化学合成制备，并立体定向地引入到乳糖操纵基因的两个位点。在d（ApApTpTpGpTpGpApGpCpGpGpApTpApApCpApApTpT）的片段I和d（ApApTpTpGpTpTpApTpCpCpGpCpTpCpApCpApApTpT）的片段II中的这些位点用p表示。将每个含有手性甲基膦酸酯的片段与互补的未修饰片段退火。通过硝酸纤维素滤膜结合试验分析这四种修饰的乳糖操纵基因与乳糖阻遏物的相互作用。在片段II中引入任何一种手性膦酸酯对阻遏物-操纵基因复合物的稳定性影响很小。当甲基膦酸酯引入片段I时，乳糖阻遏物对修饰的操纵基因的亲和力显示取决于甲基膦酸酯的立体化学构型。
