滤膜支持的亚磷酸酯法寡核苷酸合成。
Filter disc supported oligonucleotide synthesis by the phosphite method.
作者信息
Ott J, Eckstein F
出版信息
Nucleic Acids Res. 1984 Dec 11;12(23):9137-42. doi: 10.1093/nar/12.23.9137.
Abstract
The phosphite approach to oligodeoxynucleotide synthesis has been applied to the filter disc support. The method is exemplified here by the preparation of eight decadeoxynucleotides.
摘要
亚磷酸酯法合成寡脱氧核苷酸已应用于滤盘载体。本文以制备8种十聚脱氧核苷酸为例来说明该方法。
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本文引用的文献
1
Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.在微尺度上同时快速化学合成超过一百个寡核苷酸。
EMBO J. 1984 Apr;3(4):801-5. doi: 10.1002/j.1460-2075.1984.tb01888.x.
2
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.通过磷酸三酯中间体合成寡核苷酸的新有效方法。
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
3
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。
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4
Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.用于合成寡脱氧核糖核苷酸的亚磷酰胺方法的改进。
Nucleic Acids Res. 1983 May 11;11(9):2575-84. doi: 10.1093/nar/11.9.2575.
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