乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
lac repressor-lac operator interaction: NMR observations.
作者信息
Nick H, Arndt K, Boschelli F, Jarema M A, Lillis M, Sadler J, Caruthers M, Lu P
出版信息
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
Abstract
We show here the changes in the NMR spectra of the Escherichia coli lac repressor when bound to isolated lac operator DNA. The observations focus on the aromatic residues--four tyrosines and a single histidine--in the amino-terminal DNA binding domain of the lac repressor. There is a good correlation between chemical shift changes seen by 19F NMR when compared with 1 H NMR of otherwise identical repressor--DNA complexes. The results suggest that the tyrosines do not intercalate in the DNA. The NMR spectral changes with similarly sized DNA fragments, not containing the lac operator DNA sequence, are different. Thus, the amino-terminal domain of the lac repressor is independently capable of discriminating between lac operator and nonspecific DNA sequences. There can be two amino-terminal fragments per operator in the specific complex.
摘要
我们在此展示了大肠杆菌乳糖阻遏物与分离出的乳糖操纵基因DNA结合时核磁共振（NMR）谱的变化。观察聚焦于乳糖阻遏物氨基末端DNA结合结构域中的芳香族残基——四个酪氨酸和一个组氨酸。与其他条件相同的阻遏物-DNA复合物的1H NMR相比，19F NMR观察到的化学位移变化之间存在良好的相关性。结果表明酪氨酸并不嵌入DNA中。与不含乳糖操纵基因DNA序列但大小相似的DNA片段结合时，NMR谱的变化不同。因此，乳糖阻遏物的氨基末端结构域能够独立区分乳糖操纵基因和非特异性DNA序列。在特异性复合物中，每个操纵基因可能有两个氨基末端片段。
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本文引用的文献
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An amino-terminal fragment of lac repressor binds specifically to lac operator.乳糖阻遏物的氨基端片段特异性结合乳糖操纵基因。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851-4. doi: 10.1073/pnas.75.12.5851.
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