Eikmann T, Horning B, Pieler J, Riedel F N
Zentralbl Bakteriol Mikrobiol Hyg B. 1984 Mar;179(1):44-55.
In eight hospitals the quantity of microorganisms in the supply air of air conditioning systems was determined. A Reuter-Centrifugal-Sampler (by Biotest, Frankfurt) sampled 160 litres of air in four minutes and blew the particles with microorganisms on a culture medium (GKA-medium, by Biotest, Frankfurt). The Agar was breeded for 48 hours at 37 degrees C. The colonic-forming-units were counted and transformed to a volume of a cubic meter (CFU/m3). - In other publications you can find determinations of microorganisms in the supply air for operating theatres up to 15 CFU/m3. We found up to 40 CFU/m3 in the supply air after three step filtration (with an high efficiency filter) and 20-85 CFU/m3 after two step filtration. - The recommendations of other authors and some directives say that in the supply air up to 10 CFU/m3 are acceptable. According to our experiences the supply air should not contain more than 10 CFU/m3 after a 3-step-filtration and not more than 50 CFU/m3 after a 2-step-filtration. Otherwise the function of the air conditioning system should be controlled and the maintenance, too. - We think that according to the different requirements in hygiene a supply air with less than 10 CFU/m3 in the mean allows an unlimited use of the air conditioned room, while mean values up to 50 CFU/m3 are acceptable for normal operating rooms and other rooms, except for operating rooms with special hygienic requirements as for example for transplantation, arthroplasty, heart-operations (corresponds to II. and III. classification of rooms, DIN 1946/4). - Values up to 150 CFU/m3 are acceptable for rooms with normal hygienic requirements for supply air (III. classification of rooms, DIN 1946/4) and for labour-, intensive-care, newborn- and infant rooms.
在八家医院中，对空调系统送风的微生物数量进行了测定。使用Reuter离心采样器（由德国美因河畔法兰克福的Biotest公司生产）在四分钟内采集160升空气，并将带有微生物的颗粒吹到培养基（GKA培养基，由德国美因河畔法兰克福的Biotest公司生产）上。琼脂在37摄氏度下培养48小时。计算菌落形成单位并换算成立方米体积（CFU/m³）。——在其他出版物中，你可以找到手术室送风微生物含量测定结果，高达15 CFU/m³。经过三级过滤（使用高效过滤器）后，我们发现送风微生物含量高达40 CFU/m³，经过两级过滤后为20 - 85 CFU/m³。——其他作者的建议和一些指令规定，送风微生物含量高达10 CFU/m³是可以接受的。根据我们的经验，经过三级过滤后，送风微生物含量不应超过10 CFU/m³，经过两级过滤后不应超过50 CFU/m³。否则，应控制空调系统的功能并进行维护。——我们认为，根据不同的卫生要求，平均微生物含量低于10 CFU/m³的送风可允许无限制地使用空调房间，而对于普通手术室和其他房间，除了有特殊卫生要求的手术室，如移植、关节置换、心脏手术（对应DIN 1946/4标准中的II级和III级房间），平均微生物含量高达50 CFU/m³是可以接受的。——对于送风有正常卫生要求的房间（DIN 1946/4标准中的III级房间）以及产房、重症监护室、新生儿室和婴儿室，微生物含量高达150 CFU/m³是可以接受的。
