Eikmann T, Pieler J, Mathies R, Einbrodt H J
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1986 May;182(3):254-66.
In the following investigation, air-germ measurements were conducted--using the Reuter-Centrifugal-Sampler (RCS) and the Sartorius-Sampler MD 3 (SAR), and using standardized nutrient-substratum and breeding conditions--in order to detect differences in the measuring process itself. The aim of the study was to find a standard for routine-measurements in hospitals. A special "Supply-Air-Box" was developed because of the known turbulences during the emission of air from the channel; in order to cut off the interference-factor during the measurements caused by the blending with the room-air. The results of the air-germ measurements showed a sufficient reproducability and a good representation of the results in differing measuring positions with both devices during repeated measurements. A marked difference between the two measuring-devices are the markedly higher CFU-numbers in the measurements using the RCS-device and the resulting higher variability of the single-values. The measurements using the supply-air-box show that--with the usual measuring position immediately in front of the supply-air grille screen or without grille screen at the channel end--a considerable part of room-air is also registered during the determination of the CFU-number because of the existing turbulences. Because of the markedly lower CFU-numbers with this procedure, new recommendations were suggested for the maximal mean CFU-number in the supply-air. Accordingly, not more than 5 CFU/m3 of air should be found with three-step-filtered supply-air in well-maintained installations for the inrestricted utilization of the rooms, and not more than a mean value of 30 CFU/m3 of air with two-step-filtered supply-air.
在以下研究中，使用路透社离心采样器（RCS）和赛多利斯采样器MD 3（SAR），并采用标准化的营养基质和培养条件进行空气微生物测量，以检测测量过程本身的差异。该研究的目的是找到医院常规测量的标准。由于已知从通道排放空气时会产生湍流，因此开发了一种特殊的“送风箱”，以消除测量过程中与室内空气混合所造成的干扰因素。空气微生物测量结果表明，在重复测量过程中，两种设备在不同测量位置的测量结果都具有足够的可重复性和良好的代表性。两种测量设备之间的一个显著差异是，使用RCS设备进行测量时的菌落形成单位（CFU）数量明显更高，且单个值的变异性也更高。使用送风箱进行的测量表明，在通常的测量位置，即紧挨着送风格栅筛网或通道末端没有格栅筛网的情况下，由于存在湍流，在确定CFU数量时也会记录相当一部分室内空气。由于采用此程序时CFU数量明显较低，因此针对送风空气中的最大平均CFU数量提出了新的建议。因此，对于维护良好的设施中可供无限制使用房间的经过三级过滤的送风，每立方米空气中的CFU不应超过5个；对于经过二级过滤的送风，每立方米空气中的CFU平均值不应超过30个。
