Renault G, Raynal E, Sinet M, Muffat-Joly M, Berthier J P, Cornillault J, Godard B, Pocidalo J J
Am J Physiol. 1984 Apr;246(4 Pt 2):H491-9. doi: 10.1152/ajpheart.1984.246.4.H491.
A double-beam laser fluorimeter, using a single optical fiber to guide the lights, was constructed for in situ and on-line monitoring of NADH concentration [( NADH]) from normally blood-perfused living tissues. The device was tested on an isolated blood-perfused rat heart system to determine the most efficient reference wavelength for the compensation of the hemodynamic artifact induced by blood circulation in the tissues on the fluorescence measure; 586 nm was found to be an accurate reference wavelength, and a mathematical relationship was established that allowed the digital treatment of the measured fluorescence to give a signal (compensated fluorescence) that varied only with [NADH] in the volume of tissue investigated.
一种双光束激光荧光计，使用单根光纤来传导光线，被制造用于对正常血液灌注的活体组织中的烟酰胺腺嘌呤二核苷酸（NADH）浓度进行原位和在线监测。该装置在一个离体血液灌注大鼠心脏系统上进行了测试，以确定用于补偿组织中血液循环在荧光测量上引起的血液动力学伪影的最有效参考波长；发现586纳米是一个准确的参考波长，并建立了一种数学关系，使得对测量的荧光进行数字处理后能给出一个仅随所研究组织体积中的[NADH]变化的信号（补偿荧光）。
