Zurovsky Y, Sonn J
Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
J Basic Clin Physiol Pharmacol. 1992 Oct-Dec;3(4):343-58. doi: 10.1515/jbcpp.1992.3.4.343.
Most current knowledge on events in the mitochondria leading to acute renal failure originates from studies in which indirect methods were used. The disadvantage of these methods is that they cannot measure the turnover rate of various metabolites, and only one result per animal can be obtained. Chance et al. /9/ developed a method using optical techniques for continuously monitoring the fluorescence of intramitochondrial NADH, which has been applied mainly to the brain. This optical method has not yet been examined quantitatively in the kidney and no attempt has been made to adapt this method for routine measurement in kidney tissue. The purpose of this study was to adapt the surface fluorometry method for monitoring renal NADH redox state in situ, and to determine whether the hemodynamic artifacts involved in fluorometric studies of the renal surface in situ could be eliminated by using a correction factor. Another purpose was to understand the relationship between the changes in reflectance and blood volume in the rat kidney. This was achieved by measuring the reflectance after: a) blood exchange by FC-43 emulsion; b) intrarenal saline flush; c) occlusion of the renal vein, renal artery and reopening of the renal vein; d) calculation of the correlation between changes in kidney weight after renal artery occlusion, and the reflectance. Our results suggest that in the rat kidney, as opposed to the brain, a correction factor of 1:1 is not always applicable. This factor may vary between animals, and it is therefore necessary to adjust it electronically for each rat kidney. This observation contradicts the view suggesting a constant correction factor of 1:1 in the kidney. The results reported herein indicate that changes in the reflectance in the ischemic rat kidney are due to changes in blood volume. In conclusion, it seems that optical techniques for monitoring fluorescence are suitable for localized, continuous and non-invasive recording of tissue mitochondrial NADH redox states under various conditions in the rat kidney.
目前关于线粒体中导致急性肾衰竭的事件的大多数知识都源于使用间接方法的研究。这些方法的缺点是它们无法测量各种代谢物的周转率，并且每只动物只能获得一个结果。Chance等人/9/开发了一种使用光学技术连续监测线粒体内NADH荧光的方法，该方法主要应用于大脑。这种光学方法尚未在肾脏中进行定量研究，也没有人尝试将该方法应用于肾脏组织的常规测量。本研究的目的是采用表面荧光法原位监测肾脏NADH氧化还原状态，并确定是否可以通过使用校正因子消除原位肾脏表面荧光研究中涉及的血流动力学伪影。另一个目的是了解大鼠肾脏反射率变化与血容量之间的关系。这是通过在以下操作后测量反射率来实现的：a）用FC-43乳剂进行血液交换；b）肾内盐水冲洗；c）阻断肾静脉、肾动脉并重新开放肾静脉；d）计算肾动脉阻断后肾脏重量变化与反射率之间的相关性。我们的结果表明，在大鼠肾脏中，与大脑不同，1:1的校正因子并不总是适用。这个因子可能因动物而异，因此有必要对每只大鼠肾脏进行电子调整。这一观察结果与认为肾脏中校正因子恒定为1:1的观点相矛盾。本文报道的结果表明，缺血大鼠肾脏反射率的变化是由于血容量的变化。总之，似乎用于监测荧光的光学技术适用于在大鼠肾脏的各种条件下对组织线粒体NADH氧化还原状态进行局部、连续和非侵入性记录。
