Orr C S, Arthurs S C
Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas 75235-9031.
ASAIO J. 1992 Jul-Sep;38(3):M412-5. doi: 10.1097/00002480-199207000-00066.
A prototype laser-fiber optic based sensor for in situ monitoring of nicotinamide adenine dinucleotide (NADH) has been developed. This system is based on a compact neodymium-yttrium-aluminum-garnet (Nd:YAG) laser with associated harmonic generators. Light distribution to and from tissue is handled by a fiber optic network, including a long optical fiber to be inserted into the target tissue. Immobilizing the enzyme lactate dehydrogenase on the fiber tip converts the monitoring channel into a lactate sensor. A new dual beam reflection approach for blood volume artifact compensation is tested. Detection sensitivity of free NADH in the micromolar region is achieved. The method and system configuration appear feasible for continuous in situ monitoring of two important indices of ischemia and hypoxia in a clinical setting.
一种用于原位监测烟酰胺腺嘌呤二核苷酸（NADH）的基于激光光纤的传感器原型已被开发出来。该系统基于一个紧凑型钕钇铝石榴石（Nd:YAG）激光器及相关的谐波发生器。通过光纤网络来处理进出组织的光分布，该光纤网络包括一根要插入目标组织的长光纤。将乳酸脱氢酶固定在光纤尖端可将监测通道转变为乳酸传感器。测试了一种用于血容量伪影补偿的新型双光束反射方法。实现了在微摩尔区域对游离NADH的检测灵敏度。该方法和系统配置对于在临床环境中连续原位监测缺血和缺氧的两个重要指标似乎是可行的。
