Kozhechkin S N, Kuznetsova E A
Biull Eksp Biol Med. 1984 Apr;97(4):436-8.
Experiments on rabbits anesthetized with urethane and chloralose were made to study the effect of microiontophoretically applied dopamine (DA) and immobilized DA connected with a polymer (DA-P) on extracellularly recorded activity of sensorimotor cortical neurons. Both DA-P and low-molecular DA reduced the frequency of spontaneous action potentials in the majority of the examined neurons in a dose-dependent manner. The inhibitory effect of DA-P was less powerful than that of DA under the same conditions of microiontophoretic application. Since low-molecular DA included in the structure of DA-P does not penetrate the neuron, it is concluded that the specific inhibitory effect of DA on neuronal activity is determined by its interaction with receptors located on the external surface of the neuronal membrane.
对用氨基甲酸乙酯和氯醛糖麻醉的兔子进行实验，以研究微量离子导入法施加多巴胺（DA）以及与聚合物相连的固定化多巴胺（DA-P）对感觉运动皮层神经元细胞外记录活动的影响。在大多数被检测的神经元中，DA-P和低分子DA均以剂量依赖性方式降低了自发动作电位的频率。在微量离子导入应用的相同条件下，DA-P的抑制作用不如DA强大。由于DA-P结构中包含的低分子DA不能穿透神经元，因此得出结论，DA对神经元活动的特异性抑制作用是由其与位于神经元膜外表面的受体相互作用所决定的。
