Schneider R J, Weinberger C, Shenk T
Cell. 1984 May;37(1):291-8. doi: 10.1016/0092-8674(84)90325-8.
The adenovirus VAI RNA is a small polymerase III-transcribed species that is required for optimal translation of mRNAs late after infection. Mutant dl331 fails to produce this RNA species and, as a result, grows poorly. Mutant-infected cells contain normal levels of late mRNAs, but reduced levels of polypeptides are synthesized late after infection. Translational elongation occurs at normal rates in mutant, as compared to wild-type, virus-infected cells. Initiation of translation occurs with reduced efficiency in dl331 -infected cells. VAI RNA is required for formation of a stable 48S preinitiation complex and very likely functions to facilitate the interaction between 43S preinitiation complex and mRNA to form the 48S species.
腺病毒VAI RNA是一种由RNA聚合酶III转录的小分子，在感染后期mRNA的最佳翻译过程中是必需的。突变体dl331无法产生这种RNA，因此生长不良。突变体感染的细胞中晚期mRNA水平正常，但感染后期合成的多肽水平降低。与野生型病毒感染的细胞相比，突变体中的翻译延伸以正常速率发生。在dl331感染的细胞中，翻译起始效率降低。VAI RNA是形成稳定的48S起始前复合体所必需的，很可能起到促进43S起始前复合体与mRNA相互作用以形成48S复合体的作用。
