在大鼠中证明亚硝酸钠挥发性致突变性和致癌性的模型系统。

Model systems demonstrating the volatile mutagenicity and carcinogenicity of sodium nitrite in rats.

作者信息

Macdonald I A, Mader J A, Rao B G, Malatjalian D A

出版信息

Experientia. 1984 Jun 15;40(6):554-7. doi: 10.1007/BF01982327.

DOI:10.1007/BF01982327

Abstract

Volatile mutagens derived from sodium nitrite buffered at various pH values or in the presence of human feces were detected using Ames Salmonella tester strain TA 1535 on petrie plates inverted over samples. Volatile mutagenicity increased as the pH decreased and was primarily a function of the nitrous acid produced from sodium nitrite and hydrogen ions. Sodium nitrite administered intracecally to 3 Wistar rats through surgically implanted cannula caused tumors (fibrosarcoma: 1/3 and squamous cell, 2/3). The possible role of nitrite-derived mutagens in GI cancer is discussed.

摘要

使用Ames沙门氏菌测试菌株TA 1535，在倒置在样品上方的皮氏培养皿上，检测在不同pH值缓冲的亚硝酸钠或存在人类粪便的情况下产生的挥发性诱变剂。随着pH值降低，挥发性诱变性增加，这主要是由亚硝酸钠和氢离子产生的亚硝酸的作用。通过手术植入的套管向3只Wistar大鼠的盲肠内注射亚硝酸钠会导致肿瘤（纤维肉瘤：1/3，鳞状细胞癌：2/3）。讨论了亚硝酸盐衍生的诱变剂在胃肠道癌症中的可能作用。