Nerlich A G, Nerlich M L, Langer I, Demling R H
Exp Mol Pathol. 1984 Jun;40(3):311-9. doi: 10.1016/0014-4800(84)90048-0.
Acute pulmonary fibrosis following intoxication with paraquat is characterized by increased collagen synthesis and deposition in the lungs. In the present study, a chronic lung lymph fistula preparation in sheep was used to investigate the changes in procollagen type III peptide level in the lung lymph in acute pulmonary distress associated with paraquat application. The procollagen type III peptide is supposed to be an indicator of the changing biosynthetic pattern of pulmonary collagen in lung fibrosis. All animals tested showed a progressive pulmonary distress following the application of paraquat characterized by increasing lymph flows and lymph protein flows which monitored the microvascular membrane damage. In parallel to these findings, procollagen peptide levels in the lymph fluid increased, even before manifest biochemically or histologically detectable pulmonary fibrosis occurred. The present results suggest that the release of procollagen type III peptides into lymph fluid is an early indicator of beginning fibrotic tissue disarrangement, even before completed pulmonary fibrosis can be detected by common tissue analysis.
百草枯中毒后的急性肺纤维化的特征是肺中胶原蛋白合成增加和沉积。在本研究中，使用绵羊慢性肺淋巴瘘制备模型来研究在与百草枯应用相关的急性肺损伤中肺淋巴液中III型前胶原肽水平的变化。III型前胶原肽被认为是肺纤维化中肺胶原蛋白生物合成模式变化的指标。所有受试动物在应用百草枯后均出现进行性肺损伤，其特征为监测微血管膜损伤的淋巴流量和淋巴蛋白流量增加。与这些发现同时，即使在生化或组织学上可检测到的明显肺纤维化出现之前，淋巴液中的前胶原肽水平也会升高。目前的结果表明，III型前胶原肽释放到淋巴液中是纤维化组织紊乱开始的早期指标，甚至在通过常规组织分析检测到完全肺纤维化之前。
