链球菌鞭毛马达的化学修饰。
Chemical modification of Streptococcus flagellar motors.
作者信息
Conley M P, Berg H C
出版信息
J Bacteriol. 1984 Jun;158(3):832-43. doi: 10.1128/jb.158.3.832-843.1984.
Abstract
Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts to media containing low concentrations of potassium ion. Experiments were devised that distinguished reagents that lowered the proton motive force from those that blocked the generation of torque (damaged the flagellar motors). Imidazole reagents blocked the generation of torque. Amino, sulfhydryl, dithiol, and disulfide reagents did not. Some of the imidazole, amino, and sulfhydryl reagents had long-term effects on the direction of flagellar rotation.
摘要
采用视频技术记录了暴露于多种蛋白质修饰试剂下的链球菌属V4051菌株细胞运动性的变化。饥饿细胞通过单根鞭毛固定在玻璃上，用葡萄糖进行代谢供能，或用缬氨霉素处理并通过转移至含低浓度钾离子的培养基进行人工供能。设计了一些实验来区分降低质子动力的试剂和那些阻断扭矩产生（损坏鞭毛马达）的试剂。咪唑试剂阻断扭矩的产生。氨基、巯基、二硫醇和二硫键试剂则不会。一些咪唑、氨基和巯基试剂对鞭毛旋转方向有长期影响。
相似文献
1
Chemical modification of Streptococcus flagellar motors.链球菌鞭毛马达的化学修饰。
J Bacteriol. 1984 Jun;158(3):832-43. doi: 10.1128/jb.158.3.832-843.1984.
2
Isotope and thermal effects in chemiosmotic coupling to the flagellar motor of Streptococcus.化学渗透偶联到链球菌鞭毛马达中的同位素和热效应。
Cell. 1983 Mar;32(3):913-9. doi: 10.1016/0092-8674(83)90076-4.
3
A protonmotive force drives bacterial flagella.质子动力驱动细菌鞭毛。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3060-4. doi: 10.1073/pnas.74.7.3060.
4
Energy transduction in the bacterial flagellar motor. Effects of load and pH.细菌鞭毛马达中的能量转换。负载和pH值的影响。
Biophys J. 1990 Apr;57(4):779-96. doi: 10.1016/S0006-3495(90)82598-4.
5
Quantitative measurements of proton motive force and motility in Bacillus subtilis.枯草芽孢杆菌中质子动力和运动性的定量测量。
J Bacteriol. 1980 Dec;144(3):891-7. doi: 10.1128/jb.144.3.891-897.1980.
6
Constraints on flagellar rotation.鞭毛旋转的限制因素。
J Mol Biol. 1985 Aug 20;184(4):645-56. doi: 10.1016/0022-2836(85)90310-9.
7
The stall torque of the bacterial flagellar motor.细菌鞭毛马达的失速扭矩。
Biophys J. 1987 Sep;52(3):413-9. doi: 10.1016/S0006-3495(87)83230-7.
8
Flagellar rotation in the archaeon Halobacterium salinarum depends on ATP.古菌盐沼盐杆菌中的鞭毛旋转依赖于ATP。
J Mol Biol. 2008 Dec 5;384(1):1-8. doi: 10.1016/j.jmb.2008.08.057. Epub 2008 Aug 29.
9
Energetics of flagellar rotation in bacteria.细菌鞭毛旋转的能量学
J Mol Biol. 1980 Apr 15;138(3):541-61. doi: 10.1016/s0022-2836(80)80017-9.
10
Response of the flagellar rotary motor to abrupt changes in extracellular pH.鞭毛旋转马达对细胞外pH值突然变化的反应。
J Mol Biol. 1987 Feb 5;193(3):585-9. doi: 10.1016/0022-2836(87)90269-5.
引用本文的文献
1
Quenching active swarms: effects of light exposure on collective motility in swarming Serratia marcescens.淬灭活跃群体：光照对群体运动性的影响 Serratia marcescens 群体。
J R Soc Interface. 2019 Jul 26;16(156):20180960. doi: 10.1098/rsif.2018.0960. Epub 2019 Jul 17.
2
The propagation of active-passive interfaces in bacterial swarms.细菌群中主动-被动界面的传播。
Nat Commun. 2018 Dec 18;9(1):5373. doi: 10.1038/s41467-018-07781-y.
3
Differential activation of Escherichia coli chemoreceptors by blue-light stimuli.蓝光刺激对大肠杆菌化学感受器的差异激活。
J Bacteriol. 2006 Jun;188(11):3962-71. doi: 10.1128/JB.00149-06.
4
Constraints on models for the flagellar rotary motor.鞭毛旋转马达模型的限制因素。
Philos Trans R Soc Lond B Biol Sci. 2000 Apr 29;355(1396):491-501. doi: 10.1098/rstb.2000.0590.
5
Torque generated by the flagellar motor of Escherichia coli.大肠杆菌鞭毛马达产生的扭矩。
Biophys J. 1993 Nov;65(5):2201-16. doi: 10.1016/S0006-3495(93)81278-5.
6
Torque generation by the flagellar rotary motor.鞭毛旋转马达产生的扭矩。
Biophys J. 1995 Apr;68(4 Suppl):163S-166S; discussion 166S-167S.
7
Mechanical limits of bacterial flagellar motors probed by electrorotation.通过电旋转探测细菌鞭毛马达的机械极限
Biophys J. 1995 Jul;69(1):280-6. doi: 10.1016/S0006-3495(95)79900-3.
8
The stall torque of the bacterial flagellar motor.细菌鞭毛马达的失速扭矩。
Biophys J. 1987 Sep;52(3):413-9. doi: 10.1016/S0006-3495(87)83230-7.
9
Dynamics of a tightly coupled mechanism for flagellar rotation. Bacterial motility, chemiosmotic coupling, protonmotive force.鞭毛旋转紧密偶联机制的动力学。细菌运动性、化学渗透偶联、质子动力势。
Biophys J. 1989 May;55(5):905-14. doi: 10.1016/S0006-3495(89)82889-9.
10
Energy transduction in the bacterial flagellar motor. Effects of load and pH.细菌鞭毛马达中的能量转换。负载和pH值的影响。
Biophys J. 1990 Apr;57(4):779-96. doi: 10.1016/S0006-3495(90)82598-4.
本文引用的文献
1
Chemical stimulation and inhibition of bacterial motility studied with a new method.
Proc Soc Exp Biol Med. 1951 Nov;78(2):584-9. doi: 10.3181/00379727-78-19148.
2
[Kinetic studies on galactoside permease of Escherichia coli].[大肠杆菌半乳糖苷通透酶的动力学研究]
Biochim Biophys Acta. 1960 May 6;40:70-84. doi: 10.1016/0006-3002(60)91316-0.
3
On the interplay of environmental factors affecting taxis and motility in Rhodospirillum rubrum.关于影响红螺菌趋性和运动性的环境因素之间的相互作用
Arch Mikrobiol. 1958;29(2):189-212. doi: 10.1007/BF00409860.
4
Energetics of flagellar rotation in bacteria.细菌鞭毛旋转的能量学
J Mol Biol. 1980 Apr 15;138(3):541-61. doi: 10.1016/s0022-2836(80)80017-9.
5
pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate.大肠杆菌中的pH稳态：通过甲基膦酸酯和磷酸盐的31P核磁共振进行测量
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6271-5. doi: 10.1073/pnas.78.10.6271.
6
Effect of the proton electrochemical gradient on maleimide inactivation of active transport in Escherichia coli membrane vesicles.质子电化学梯度对大肠杆菌膜囊泡中主动运输的马来酰亚胺失活作用的影响。
Biochemistry. 1981 May 26;20(11):3308-13. doi: 10.1021/bi00514a050.
7
Probes of membrane potential in Escherichia coli cells.大肠杆菌细胞中膜电位的探针。
FEBS Lett. 1981 Mar 23;125(2):197-200. doi: 10.1016/0014-5793(81)80717-x.
8
Isotope and thermal effects in chemiosmotic coupling to the flagellar motor of Streptococcus.化学渗透偶联到链球菌鞭毛马达中的同位素和热效应。
Cell. 1983 Mar;32(3):913-9. doi: 10.1016/0092-8674(83)90076-4.
9
The steady-state counterclockwise/clockwise ratio of bacterial flagellar motors is regulated by protonmotive force.细菌鞭毛马达的稳态逆时针/顺时针比率受质子动力调控。
J Mol Biol. 1980 Apr 15;138(3):563-97. doi: 10.1016/s0022-2836(80)80018-0.
10
Energy coupling to potassium transport in Streptococcus faecalis. Interplay of ATP and the protonmotive force.粪肠球菌中能量与钾转运的偶联。ATP与质子动力的相互作用。
J Biol Chem. 1980 Jan 25;255(2):433-40.
Zotero 插件