Conley M P, Berg H C
J Bacteriol. 1984 Jun;158(3):832-43. doi: 10.1128/jb.158.3.832-843.1984.
Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts to media containing low concentrations of potassium ion. Experiments were devised that distinguished reagents that lowered the proton motive force from those that blocked the generation of torque (damaged the flagellar motors). Imidazole reagents blocked the generation of torque. Amino, sulfhydryl, dithiol, and disulfide reagents did not. Some of the imidazole, amino, and sulfhydryl reagents had long-term effects on the direction of flagellar rotation.
采用视频技术记录了暴露于多种蛋白质修饰试剂下的链球菌属V4051菌株细胞运动性的变化。饥饿细胞通过单根鞭毛固定在玻璃上，用葡萄糖进行代谢供能，或用缬氨霉素处理并通过转移至含低浓度钾离子的培养基进行人工供能。设计了一些实验来区分降低质子动力的试剂和那些阻断扭矩产生（损坏鞭毛马达）的试剂。咪唑试剂阻断扭矩的产生。氨基、巯基、二硫醇和二硫键试剂则不会。一些咪唑、氨基和巯基试剂对鞭毛旋转方向有长期影响。
