鸡胗肌肌球蛋白分子的电子显微镜研究III. 肌球蛋白二聚体

Electron microscopic studies on myosin molecules from chicken gizzard muscle III. Myosin dimers.

作者信息

Onishi H, Wakabayashi T

出版信息

J Biochem. 1984 Mar;95(3):903-5. doi: 10.1093/oxfordjournals.jbchem.a134686.

DOI:10.1093/oxfordjournals.jbchem.a134686

Abstract

Previously, we (Onishi, H. & Wakabayashi, T. (1982) J. Biochem. 92, 871) reported that the ATP-induced disassembly of chicken gizzard "thick filaments" resulted in myosin monomers with "looped" tails. In the present study, we found that these monomers assembled themselves into antiparallel dimers when they were placed in a medium of low ionic strength (approximately 2 mM).

摘要

此前，我们（大西浩和若林彻，（1982年）《生物化学杂志》92卷，871页）报道，ATP诱导鸡砂囊“粗肌丝”解体，产生具有“环状”尾部的肌球蛋白单体。在本研究中，我们发现，当将这些单体置于低离子强度（约2 mM）的介质中时，它们会自行组装成反平行二聚体。