Galvan M, Dörge A, Beck F, Rick R
Pflugers Arch. 1984 Mar;400(3):274-9. doi: 10.1007/BF00581559.
Intracellular element concentrations were measured in rat sympathetic neurones using energy dispersive electron microprobe analysis. The resting intracellular concentrations of sodium potassium and chloride measured in ganglia maintained for about 90 min in vitro at 25 degrees C were 3, 155 and 25 mmol/kg total tissue wet weight respectively. Recalculated in mmol/l cell water, these values are 5, 196 and 32 respectively. There were no significant differences between the nuclear and cytoplasmic values of these ions. Incubation in either carbachol (180 mumol/l, 4 min) or ouabain (1 mmol/1, 60 min) significantly increased the intracellular sodium and decreased the intracellular potassium concentrations. Neither substance materially altered the intracellular chloride concentration. The data obtained are compared and contrasted to those obtained in mammalian sympathetic neurones using chemical analysis and ion-sensitive microelectrodes.
使用能量分散电子微探针分析测量大鼠交感神经元中的细胞内元素浓度。在25℃下体外维持约90分钟的神经节中，测得的钠、钾和氯的静息细胞内浓度分别为3、155和25 mmol/kg总组织湿重。以mmol/l细胞水重新计算，这些值分别为5、196和32。这些离子的核内和胞质值之间没有显著差异。用卡巴胆碱（180 μmol/l，4分钟）或哇巴因（1 mmol/1，60分钟）孵育可显著增加细胞内钠浓度并降低细胞内钾浓度。两种物质均未实质性改变细胞内氯浓度。将获得的数据与使用化学分析和离子敏感微电极在哺乳动物交感神经元中获得的数据进行比较和对比。
