玉米线粒体26 S rRNA基因及其侧翼区域的序列分析。

Sequence analysis of the maize mitochondrial 26 S rRNA gene and flanking regions.

作者信息

Dale R M, Mendu N, Ginsburg H, Kridl J C

出版信息

Plasmid. 1984 Mar;11(2):141-50. doi: 10.1016/0147-619x(84)90019-2.

Abstract

A single copy of the large ribosomal 26 S rRNA gene is found in the maize mitochondrial genome. The sequence of this gene and the flanking regions has been determined using the M13 dideoxy sequencing method. The maize mt 26 S rDNA shares a high degree of homology with the Escherichia coli 23 S rDNA, and the approximate 5' and 3' ends of the maize 26 S rDNA have been located by comparison with the E. coli sequence. The maize mt 26 S rDNA has also been compared with the sequences of the maize chloroplast 23 S rDNA, the human mitochondrial 16 S rDNA, part of the yeast mitochondrial 21 S rDNA, and the yeast cytoplasmic 25 S rDNA. In all cases, there are numerous regions of 70% or higher homology.

摘要

在玉米线粒体基因组中发现了一个大核糖体26 S rRNA基因的单拷贝。该基因及其侧翼区域的序列已使用M13双脱氧测序法测定。玉米线粒体26 S rDNA与大肠杆菌23 S rDNA具有高度同源性，通过与大肠杆菌序列比较确定了玉米26 S rDNA的大致5'和3'末端。还将玉米线粒体26 S rDNA与玉米叶绿体23 S rDNA、人类线粒体16 S rDNA、酵母线粒体21 S rDNA的一部分以及酵母细胞质25 S rDNA的序列进行了比较。在所有情况下，都有许多同源性达70%或更高的区域。