体外受精周期中卵泡液内卵丘-卵母细胞复合体延长培养的效果
The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles.
作者信息
Wilding Martin, Singer Michael, Fehr Peter, Haeberlin Felix, Roth Felix, Lachat Remo, Di Matteo Loredana, Capobianco Clemente, Dale Brian
机构信息
Centre for Reproductive Biology, Clinica Villa del Sole, Via Manzoni 15, 80126 Naples, Italy.
出版信息
J Assist Reprod Genet. 2006 Mar;23(3):129-36. doi: 10.1007/s10815-005-9016-y. Epub 2006 Apr 19.
PURPOSE
To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles.
METHODS
A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential.
RESULTS
Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences.
CONCLUSIONS
Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures.
目的
评估在体外受精（IVF）和卵胞浆内单精子注射（ICSI）周期之前，将卵母细胞在卵泡液中预孵育的临床和生物学效应。
方法
一系列患者在取卵后将卵母细胞在患者自身卵泡液中预孵育3小时，然后按照标准方案进行处理。对照卵母细胞在正常IVF培养基中预孵育。使用荧光技术检测卵母细胞线粒体膜电位。
结果
卵母细胞在卵泡液中预孵育后，受精、妊娠和着床率均显著提高。进一步测试表明，卵泡液和人工培养基之间的pH差异可能是造成这些差异的关键因素。
结论
人卵母细胞在卵泡液中预孵育可改善IVF后的结果。这可能部分归因于体外受精过程中人工培养基使用了非“生理”pH。
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