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The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles.体外受精周期中卵泡液内卵丘-卵母细胞复合体延长培养的效果
J Assist Reprod Genet. 2006 Mar;23(3):129-36. doi: 10.1007/s10815-005-9016-y. Epub 2006 Apr 19.
2
Anti-Müllerian hormone and progesterone levels in human follicular fluid are predictors of embryonic development.人卵泡液中的抗苗勒管激素和孕酮水平是胚胎发育的预测指标。
Reprod Biol Endocrinol. 2019 Jun 19;17(1):47. doi: 10.1186/s12958-019-0492-9.
3
A pre-in vitro maturation medium containing cumulus oocyte complex ligand-receptor signaling molecules maintains meiotic arrest, supports the cumulus oocyte complex and improves oocyte developmental competence.含有卵丘卵母细胞复合物配体-受体信号分子的预体外成熟培养基可维持减数分裂阻滞，支持卵丘卵母细胞复合物，并提高卵母细胞发育能力。
Mol Hum Reprod. 2017 Sep 1;23(9):594-606. doi: 10.1093/molehr/gax032.
4
AMH has no role in predicting oocyte quality in women with advanced age undergoing IVF/ICSI cycles.抗缪勒管激素在预测高龄行体外受精/卵胞浆内单精子注射周期妇女卵母细胞质量方面没有作用。
Sci Rep. 2020 Nov 12;10(1):19750. doi: 10.1038/s41598-020-76543-y.
5
Oocyte matched follicular fluid anti-Müllerian hormone is an excellent predictor of live birth after fresh single embryo transfer.卵母细胞匹配的卵泡液抗苗勒管激素是新鲜单胚胎移植后活产的优秀预测指标。
Hum Reprod. 2019 Nov 1;34(11):2244-2253. doi: 10.1093/humrep/dez186.
6
[The follicular flushing duplicate the pregnancy rate on semi natural cycle IVF].
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7
[The effect of follicular fluid pH on the quality and number of oocytes produced in the in vitro fertilization program].
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Inhibin B predicts oocyte number and the ratio IGF-I/IGFBP-1 may indicate oocyte quality during ovarian hyperstimulation for in vitro fertilization.抑制素B可预测卵母细胞数量，而胰岛素样生长因子-I/胰岛素样生长因子结合蛋白-1的比值可能在体外受精的卵巢过度刺激过程中指示卵母细胞质量。
J Assist Reprod Genet. 2003 May;20(5):167-76. doi: 10.1023/a:1023656225053.
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Dimeric inhibins and activin A in human follicular fluid and oocyte-cumulus culture medium.人卵泡液和卵母细胞-卵丘复合体培养基中的二聚体抑制素和激活素A
Hum Reprod. 1999 Oct;14(10):2525-30. doi: 10.1093/humrep/14.10.2525.
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Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality.体外受精过程中，在采集卵母细胞时人卵丘细胞中孕酮受体表达的降低与良好的胚胎质量相关。
Hum Reprod. 2005 Aug;20(8):2194-200. doi: 10.1093/humrep/dei005. Epub 2005 Mar 31.

本文引用的文献

1
Serum and follicular fluid leptin levels in patients undergoing controlled ovarian hyperstimulation for in vitro fertilization cycle.
Clin Exp Obstet Gynecol. 2004;31(2):103-6.
2
The pH of the human uterine cavity in situ.人体子宫腔内原位的pH值。
Am J Obstet Gynecol. 1955 Jul;70(1):60-64. doi: 10.1016/0002-9378(55)90288-x.
3
Cortisol and cortisone in human follicular fluid and serum and the outcome of IVF treatment.
Hum Reprod. 2003 Aug;18(8):1613-7. doi: 10.1093/humrep/deg352.
4
Scavenger capacity of follicular fluid, decidua and culture medium with regard to assisted reproduction: an in vitro study using electron paramagnetic resonance spectroscopy.卵泡液、蜕膜及培养基在辅助生殖方面的清除能力：一项使用电子顺磁共振波谱的体外研究
Gynecol Obstet Invest. 2003;55(3):178-82. doi: 10.1159/000071534.
5
High outcome predictability after IVF using a combined score for zygote and embryo morphology and growth rate.使用受精卵和胚胎形态及生长率综合评分进行体外受精后具有高结局可预测性。
Hum Reprod. 2002 Sep;17(9):2402-9. doi: 10.1093/humrep/17.9.2402.
6
Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality.体外受精患者卵泡液和血清中肌醇的浓度：与卵母细胞质量的关系。
Hum Reprod. 2002 Jun;17(6):1591-6. doi: 10.1093/humrep/17.6.1591.
7
Follicular fluid markers of oocyte developmental potential.卵母细胞发育潜能的卵泡液标志物。
Hum Reprod. 2002 Apr;17(4):1017-22. doi: 10.1093/humrep/17.4.1017.
8
Vascular endothelial growth factor, platelet endothelial cell adhesion molecule-1 and vascular cell adhesion molecule-1 in the follicular fluid of patients undergoing IVF.体外受精患者卵泡液中的血管内皮生长因子、血小板内皮细胞黏附分子-1和血管细胞黏附分子-1
Hum Reprod. 2001 Jul;16(7):1376-81. doi: 10.1093/humrep/16.7.1376.
9
The CONSORT statement: revised recommendations for improving the quality of reports of parallel-group randomised trials.《CONSORT 声明：改进平行组随机试验报告质量的修订建议》
Lancet. 2001 Apr 14;357(9263):1191-4.
10
The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization.卵泡液活性氧对体外受精结局的影响。
Int J Fertil Womens Med. 2000 Sep-Oct;45(5):314-20.

体外受精周期中卵泡液内卵丘-卵母细胞复合体延长培养的效果

The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles.

作者信息

Wilding Martin, Singer Michael, Fehr Peter, Haeberlin Felix, Roth Felix, Lachat Remo, Di Matteo Loredana, Capobianco Clemente, Dale Brian

机构信息

Centre for Reproductive Biology, Clinica Villa del Sole, Via Manzoni 15, 80126 Naples, Italy.

出版信息

J Assist Reprod Genet. 2006 Mar;23(3):129-36. doi: 10.1007/s10815-005-9016-y. Epub 2006 Apr 19.

DOI:10.1007/s10815-005-9016-y
PMID:16622803
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455035/
Abstract

PURPOSE

To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles.

METHODS

A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential.

RESULTS

Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences.

CONCLUSIONS

Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures.

摘要

目的

评估在体外受精（IVF）和卵胞浆内单精子注射（ICSI）周期之前，将卵母细胞在卵泡液中预孵育的临床和生物学效应。

方法

一系列患者在取卵后将卵母细胞在患者自身卵泡液中预孵育3小时，然后按照标准方案进行处理。对照卵母细胞在正常IVF培养基中预孵育。使用荧光技术检测卵母细胞线粒体膜电位。

结果

卵母细胞在卵泡液中预孵育后，受精、妊娠和着床率均显著提高。进一步测试表明，卵泡液和人工培养基之间的pH差异可能是造成这些差异的关键因素。

结论

人卵母细胞在卵泡液中预孵育可改善IVF后的结果。这可能部分归因于体外受精过程中人工培养基使用了非“生理”pH。