Hammond K, Reboiras M D, Lyle I G, Jones M N
Biochim Biophys Acta. 1984 Jul 11;774(1):19-25. doi: 10.1016/0005-2736(84)90269-4.
Phosphatidylinositol (PI), dipalmitoylphosphatidylcholine (DPPC) and mixed lipid (DPPC plus PI) sonicated vesicles have been prepared covering a range of composition. The vesicles were characterised by gel filtration, electron microscopy and photon correlation spectroscopy. The dimensions of the vesicles as measured by electron microscopy were in good accord with those obtained from photon correlation spectroscopy measurements. The number average diameters of the vesicles increase on increasing the PI content and range from approx. 30-80 nm as the weight % of PI is increased from 0 to 100. Gel filtration on Sepharose 4B columns gave anomalous results indicating that PI-containing vesicles were retarded on the gel possibly due to an interaction between the inositol headgroup and the gel matrix. Electrophoretic measurements on multilamellar vesicles show that the surface charge density increases with the PI content of the vesicles upto 50 weight % PI and remains constant thereafter. The radii of sonicated vesicles also increase with PI content which reflects a decreasing liposome curvature with increasing surface charge density.
已制备了一系列不同组成的磷脂酰肌醇（PI）、二棕榈酰磷脂酰胆碱（DPPC）以及混合脂质（DPPC加PI）超声处理囊泡。通过凝胶过滤、电子显微镜和光子相关光谱对囊泡进行了表征。通过电子显微镜测量的囊泡尺寸与通过光子相关光谱测量获得的尺寸高度一致。随着PI含量的增加，囊泡的数均直径增大，当PI的重量百分比从0增加到100时，直径范围约为30 - 80纳米。在琼脂糖4B柱上进行凝胶过滤得到了异常结果，表明含PI的囊泡在凝胶上受阻，这可能是由于肌醇头部基团与凝胶基质之间的相互作用。对多层囊泡的电泳测量表明，表面电荷密度随着囊泡中PI含量的增加而增加，直至PI含量达到50重量%，此后保持恒定。超声处理囊泡的半径也随着PI含量的增加而增大，这反映出随着表面电荷密度的增加，脂质体曲率减小。
