Michna H
Cell Tissue Res. 1984;236(2):465-70. doi: 10.1007/BF00214251.
This study was designed to gain more detailed morphological information on skeletal tendons in the course of adaptation to physical loading. The effect on collagen fibrils was investigated in 6-week-old mice by means of electron microscopy. Physical loading was performed on a treadmill 5 days a week for 1, 3, 5, 7 and 10 weeks. Morphometric analysis of collagen fibrils revealed the mean diameter, the diameter distribution, the number and the cross-sectional area. The principal observations included: 1. After one week of physical loading an increase in mean fibril diameter (30%, p less than or equal to 0.01), in number (15%, p less than or equal to 0.05), and in cross-sectional area (15%, p less than or equal to 0.05), as well as a change in mean fibril diameter distribution. 2. From the third to the seventh week a fall under the level of the controls in mean diameter (26%, p less than or equal to 0.01), in number (26%, p less than or equal to 0.01), and a reduced cross-sectional area (17%, p less than or equal to 0.01), accompanied by signs of splitting of individual collagen fibrils. 3. In the long-term study an increase in fibril number (29%, p less than or equal to 0.01), a fall in mean diameter from 189 nm in the controls to 179 nm (p less than or equal to 0.05) but no statistically significant change in the relative cross-sectional area (32%) per unit in comparison to unloaded tendons. The possible physiological implications of the findings are discussed in the light of several regulatory mechanisms known to appear during the course of physical loading in connective tissues.
本研究旨在获取更多关于骨骼肌腱在适应体力负荷过程中的详细形态学信息。通过电子显微镜研究了6周龄小鼠胶原纤维的变化情况。每周5天在跑步机上进行体力负荷训练，持续1、3、5、7和10周。对胶原纤维进行形态计量分析，得出平均直径、直径分布、数量和横截面积。主要观察结果包括：1. 体力负荷训练1周后，平均纤维直径增加（30%，p≤0.01）、数量增加（15%，p≤0.05）、横截面积增加（15%，p≤0.05），同时平均纤维直径分布发生变化。2. 从第3周到第7周，平均直径（26%，p≤0.01）、数量（26%，p≤0.01）低于对照组水平，横截面积减小（17%，p≤0.01），同时伴有个别胶原纤维分裂的迹象。3. 在长期研究中，纤维数量增加（29%，p≤0.01），平均直径从对照组的189纳米降至179纳米（p≤0.05），但与未负荷肌腱相比，单位相对横截面积（32%）无统计学显著变化。结合结缔组织在体力负荷过程中已知出现的几种调节机制，讨论了这些发现可能的生理意义。
