Allardyce R A, Wilson A
Clin Allergy. 1984 May;14(3):259-67. doi: 10.1111/j.1365-2222.1984.tb02205.x.
Breast milk cell culture supernatants from atopic mothers (nineteen) were compared by ELISA with normal breast-feeding controls (twenty-one) for regulation of cord blood lymphocyte IgA and IgE secretion in vitro. A minority of atopic (five out of nineteen) and normal (three out of twenty-one) cell supernatants stimulated cord blood lymphocyte IgA release to the same extent. The others were inactive. Stimulation was not related to breast milk cells donor atopic history or cord blood lymphocyte atopic heredity. In contrast, 70% of atopic milk cell supernatants stimulated cord blood lymphocyte cultures to form IgE (means +/- s.d. = 2070 +/- 2240 pg/culture) while stimulatory supernatants (24%) from normal donors resulted in less lymphocyte IgE release (means +/- s.d. = 680 +/- 490 pg/culture) (p less than 0.001). These differences did not correlate with breast milk cell supernatant IgE concentrations, cord blood donor serum IgE levels or atopic heredity.
通过酶联免疫吸附测定法（ELISA），比较了来自特应性母亲（19名）的母乳细胞培养上清液与正常母乳喂养对照组（21名）的母乳细胞培养上清液对体外脐血淋巴细胞IgA和IgE分泌的调节作用。少数特应性（19名中有5名）和正常（21名中有3名）细胞上清液对脐血淋巴细胞IgA释放的刺激程度相同。其他则无活性。刺激作用与母乳细胞供体的特应性病史或脐血淋巴细胞的特应性遗传无关。相比之下，70%的特应性母乳细胞上清液刺激脐血淋巴细胞培养物产生IgE（平均值±标准差=2070±2240 pg/培养物），而正常供体的刺激性上清液（24%）导致淋巴细胞IgE释放较少（平均值±标准差=680±490 pg/培养物）（p<0.001）。这些差异与母乳细胞上清液IgE浓度、脐血供体血清IgE水平或特应性遗传无关。
