Stephens S, Duffy S W, Page C
Clin Exp Immunol. 1986 Aug;65(2):396-400.
Production of gamma-interferon (gamma-IFN) in vitro by peripheral blood mononuclear cells (PBMC) from 15 breast-fed and 15 bottle-fed infants has been studied from birth to 9 months of age and compared with production by adult cells. Using a Terasaki plate microculture system with serum-free medium, PBMC were stimulated with staphylococcal enterotoxin A (SEA) and gamma-IFN production was assessed by an immunoradiometric assay. Cord blood mononuclear cells (CBMC) and PBMC from all infants secreted large quantities of gamma-IFN. The levels secreted did not change significantly with age over the 9 months of the study, nor did they differ from the levels secreted by adult cells. Cells from the bottle-fed infants secreted slightly more gamma-IFN than cells from breast-fed infants, but this difference was not significant. These results indicate that the potential for PBMC to secrete gamma-IFN in vitro is fully developed at birth in full-term infants and cannot therefore be further influenced by subsequent breast- or bottle-feeding. In addition, the greater susceptibility of infants than adults to certain bacterial and viral infections cannot be attributed to a deficiency in the potential of infant cells to secrete gamma-IFN in vitro.
对15名母乳喂养婴儿和15名人工喂养婴儿从出生到9个月大的外周血单个核细胞（PBMC）体外产生γ干扰素（γ-IFN）的情况进行了研究，并与成人细胞的产生情况进行了比较。使用无血清培养基的Terasaki板微量培养系统，用葡萄球菌肠毒素A（SEA）刺激PBMC，并通过免疫放射测定法评估γ-IFN的产生。所有婴儿的脐血单个核细胞（CBMC）和PBMC都分泌大量的γ-IFN。在9个月的研究期间，分泌水平并未随年龄显著变化，也与成人细胞的分泌水平没有差异。人工喂养婴儿的细胞分泌的γ-IFN略多于母乳喂养婴儿的细胞，但这种差异并不显著。这些结果表明，足月婴儿出生时PBMC体外分泌γ-IFN的潜力已完全发育，因此随后的母乳喂养或人工喂养不会进一步影响它。此外，婴儿比成人更容易感染某些细菌和病毒，这不能归因于婴儿细胞体外分泌γ-IFN的潜力不足。
