人类金属硫蛋白基因家族：结构与表达
The human metallothionein gene family: structure and expression.
作者信息
Karin M, Richards R I
出版信息
Environ Health Perspect. 1984 Mar;54:111-5. doi: 10.1289/ehp.8454111.
Abstract
Metallothioneins (MTs) are encoded by a multigene family in man. We have isolated those genes and analyzed the structure of some of them. The MT-II variant is encoded by a single functional gene: MT-IIA. The MT-IIB gene is a processed pseudogene derived from a reverse transcript of MT-II mRNA. On the other hand, the MT-I class of variants are encoded by a large number of genes, arranged in tandem. The MT-IIA and the MT-IA genes show a differential response to glucocorticoid hormones and heavy metals, yet they are both expressed in primary human fibroblasts and in HeLa cells. Expression of both of those genes, in high level after transfer on bovine papilloma virus vectors, leads to increased resistance of the host cells to cadmium-induced toxicity.
摘要
金属硫蛋白（MTs）由人类的一个多基因家族编码。我们已分离出这些基因并分析了其中一些基因的结构。MT-II变体由单个功能基因MT-IIA编码。MT-IIB基因是一个由MT-II mRNA反转录而来的加工假基因。另一方面，MT-I类变体由大量串联排列的基因编码。MT-IIA和MT-IA基因对糖皮质激素和重金属表现出不同的反应，但它们在原代人成纤维细胞和HeLa细胞中均有表达。在转移到牛乳头瘤病毒载体上后，这两个基因高水平表达会导致宿主细胞对镉诱导的毒性的抗性增加。
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本文引用的文献
1
2
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Nature. 1980 Jul 17;286(5770):295-7. doi: 10.1038/286295a0.
3
Induction of metallothionein mRNA in HeLa cells by dexamethasone and by heavy metals.地塞米松和重金属对HeLa细胞中金属硫蛋白mRNA的诱导作用。
Eur J Biochem. 1981 Sep 1;118(3):527-31. doi: 10.1111/j.1432-1033.1981.tb05551.x.
4
Transcriptional regulation of the mouse metallothionein-I gene by heavy metals.重金属对小鼠金属硫蛋白-I基因的转录调控
J Biol Chem. 1981 Jun 10;256(11):5712-6.
5
Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids.糖皮质激素对小鼠肝脏金属硫蛋白-I基因的转录调控
Nature. 1981 May 28;291(5813):340-2. doi: 10.1038/291340a0.
6
Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells.糖皮质激素对培养的小鼠细胞中金属硫蛋白-I mRNA 合成的调节。
J Biol Chem. 1981 Mar 25;256(6):2621-4.
7
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8
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9
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