van Putten J, van de Ruit M, Beunis M, Hemker H C
Haemostasis. 1984;14(2):184-94. doi: 10.1159/000215055.
Spectrophotometric heparin assays which are based on the catalytic effect of heparin on either the inactivation of thrombin or that of factor Xa by antithrombin III, were adapted for use in a laboratory batch analyzer. Optimal conditions were determined for assays using the chromogenic substrates Chromozym-Th and S-2238 with thrombin, and S-2222 with factor Xa. Inactivation of the clotting enzyme by antithrombin III was stopped by addition of chromogenic substrate. Assays thus obtained appeared to be applicable in a wider range of heparin concentrations and were less dependent on plasma antithrombin III concentration that known manual spectrophotometric methods. The best results were obtained with the methods based on thrombin inactivation and applying a logarithmic reference curve.
基于肝素对抗凝血酶III使凝血酶或因子Xa失活的催化作用的分光光度法肝素测定法，被应用于实验室批量分析仪。确定了使用生色底物Chromozym-Th和S-2238与凝血酶，以及S-2222与因子Xa进行测定的最佳条件。通过添加生色底物来终止抗凝血酶III对凝血酶的失活作用。由此获得的测定法似乎适用于更广泛的肝素浓度范围，并且比已知的手工分光光度法更少依赖血浆抗凝血酶III浓度。基于凝血酶失活并应用对数参考曲线的方法获得了最佳结果。
