Wagenvoord R J, Hendrix H H, Kolde H J, Hemker H C
Department of Biochemistry, University of Limburg, Maastricht, The Netherlands.
Haemostasis. 1993;23(1):26-37. doi: 10.1159/000216849.
A new sensitive chromogenic heparin assay is developed, which is well suited for clinical use. For the assay two reaction mixtures are required which can be lyophilized and reconstituted on the day of use. These reagents are stable during at least 6 h. Only two time-dependent pipetting steps are necessary. Any compound that inactivates thrombin, or can potentiate thrombin inactivation by an inhibitor, can be measured with this assay, including standard heparin, low molecular weight heparins, hirudin, alpha-NAPAP, pentosan polysulphate and dermatan sulphate. It is shown that heparin can be measured accurately in whole blood and in plasma. By addition of dextran sulphate to one of the reagents a platelet factor 4-insensitive assay is developed, so heparin can be measured even in blood that is partially activated and thus contains platelet factor 4 which neutralizes heparin.
开发了一种新型灵敏的显色肝素测定法，非常适合临床使用。该测定法需要两种反应混合物，它们可以冻干并在使用当天复溶。这些试剂至少在6小时内稳定。仅需两个与时间相关的移液步骤。任何能使凝血酶失活或可增强抑制剂对凝血酶失活作用的化合物都可以用该测定法进行测量，包括标准肝素、低分子量肝素、水蛭素、α-萘丙酰基精氨酸乙酯、戊聚糖多硫酸盐和硫酸皮肤素。结果表明，肝素可以在全血和血浆中准确测量。通过向其中一种试剂中添加硫酸葡聚糖，开发了一种对血小板因子4不敏感的测定法，因此即使在部分激活的血液中（因而含有中和肝素的血小板因子4）也可以测量肝素。
