Zapevalova N P
Int J Pept Protein Res. 1984 May;23(5):528-34. doi: 10.1111/j.1399-3011.1984.tb02754.x.
The synthesis of peptides in the presence of papain at pH 8-9.5 is described. Starting substances are acylamino acid alkyl esters (the carboxyl component) and amides or tert.-butylesters of amino acids, as well as peptide (the amino component). Under such conditions secondary hydrolysis is not essential, making the synthesis of peptides soluble in aqueous medium. The yield of peptides is 50-94%. The effect of different factors (temperature, solvents, reagent concentrations) on the result of the reaction has been studied. It has been found that an excess of the carboxyl component is expedient to increase the yield of peptides.
描述了在pH 8 - 9.5条件下木瓜蛋白酶存在时肽的合成。起始物质是酰基氨基酸烷基酯（羧基组分）、氨基酸的酰胺或叔丁酯以及肽（氨基组分）。在这种条件下，二次水解不是必需的，这使得可在水性介质中合成可溶性肽。肽的产率为50 - 94%。研究了不同因素（温度、溶剂、试剂浓度）对反应结果的影响。已发现过量的羧基组分有利于提高肽的产率。
