Walla M D, Lau P Y, Morgan S L, Fox A, Brown A
J Chromatogr. 1984 Apr 24;288(2):399-413. doi: 10.1016/s0021-9673(01)93716-1.
Capillary gas chromatography using fused-silica columns followed by electron impact or chemical ionization mass spectrometry was used to profile and identify neutral and amino sugars present in several legionellae and other bacteria. A modified alditol acetate derivatization method was employed to produce volatile carbohydrate derivatives. Muramic acid, a component of bacterial peptidoglycan, was detected in all legionellae examined. Heptose, a component of bacterial lipopolysaccharide, was identified in Escherichia coli organisms and in several purified Gram-negative bacterial lipopolysaccharides but not in the legionellae examined. Two amino dideoxyhexoses were found to be present in several of the Legionellae examined. The potential of gas chromatography-mass spectrometry for the direct chemical characterization of microorganisms is discussed.
使用熔融石英柱的毛细管气相色谱法，随后采用电子轰击或化学电离质谱法，对几种军团菌和其他细菌中存在的中性糖和氨基糖进行分析和鉴定。采用改良的乙酰糖醇乙酸酯衍生化方法制备挥发性碳水化合物衍生物。在所有检测的军团菌中均检测到细菌肽聚糖的组成成分——胞壁酸。在大肠杆菌和几种纯化的革兰氏阴性菌脂多糖中鉴定出细菌脂多糖的组成成分——庚糖，但在所检测的军团菌中未发现。在几种检测的军团菌中发现存在两种氨基二脱氧己糖。讨论了气相色谱 - 质谱联用技术直接对微生物进行化学表征的潜力。
