胎鼠的视交叉上核：使用¹⁴C标记的脱氧葡萄糖对功能性昼夜节律时钟的表征
The suprachiasmatic nuclei of the fetal rat: characterization of a functional circadian clock using 14C-labeled deoxyglucose.
作者信息
Reppert S M, Schwartz W J
出版信息
J Neurosci. 1984 Jul;4(7):1677-82. doi: 10.1523/JNEUROSCI.04-07-01677.1984.
The circadian clock located in the suprachiasmatic nuclei (SCN) was characterized in the fetal rat by using 14C-labeled deoxyglucose to monitor glucose utilization (metabolic activity) of the nuclei. A clear day-night oscillation of metabolic activity was detectable in the fetal SCN from the 19th through the 21st days of gestation; the nuclei were metabolically active during the subjective day and metabolically inactive during the subjective night. During the subjective day on gestational day 21, the fetal SCN were found to manifest high metabolic activity for most of the subjective day. We were able to acutely dissociate SCN metabolic activity in the mother rat from that in the fetus by exposing the pregnant animals to light during the normal dark period of diurnal lighting on gestational day 20. The results show the utility of the deoxyglucose method for directly investigating prenatally the function of the biological clock located in the SCN.
通过使用14C标记的脱氧葡萄糖来监测视交叉上核（SCN）的葡萄糖利用情况（代谢活性），对胎鼠体内的生物钟进行了表征。在妊娠第19天至21天的胎鼠SCN中，可检测到明显的昼夜代谢活性振荡；这些核在主观白天具有代谢活性，而在主观夜间则无代谢活性。在妊娠第21天的主观白天期间，发现胎鼠SCN在大部分主观白天表现出高代谢活性。通过在妊娠第20天正常昼夜光照的黑暗期将怀孕动物暴露于光线下，我们能够使母鼠的SCN代谢活性与胎儿的SCN代谢活性急性分离。结果表明，脱氧葡萄糖法可用于直接在产前研究位于SCN中的生物钟的功能。
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