年龄对C57B1/6J小鼠脑、肝、肾和骨骼肌提取物中肽链起始和延伸的影响。

Effect of age on peptide chain initiation and elongation in preparations from brain, liver, kidney and skeletal muscle of the C57B1/6J mouse.

作者信息

Blazejowski C A, Webster G C

出版信息

Mech Ageing Dev. 1984 Jun;25(3):323-33. doi: 10.1016/0047-6374(84)90005-8.

Abstract

The effects of age on the initiation and elongation stages of protein synthesis were measured in cell-free preparations from brain, liver, kidney and skeletal muscle of young (3-5 months) and senescent (23-27 months) female C57B1/6J mice. The ability to form initiation complexes from isolated 40 S and 60 S ribosomal subunits decreased only slightly with age. In contrast, the rate of peptide chain elongation decreased by 67% in brain preparations, 80% in liver, 81% in kidney and 85% in skeletal muscle of the senescent mice when compared with the young mice.

摘要

在年轻（3 - 5个月）和衰老（23 - 27个月）的雌性C57B1/6J小鼠的脑、肝、肾和骨骼肌的无细胞制剂中，测量了年龄对蛋白质合成起始和延伸阶段的影响。从分离的40S和60S核糖体亚基形成起始复合物的能力仅随年龄略有下降。相比之下，与年轻小鼠相比，衰老小鼠脑制剂中的肽链延伸速率下降了67%，肝中下降了80%，肾中下降了81%，骨骼肌中下降了85%。