马铃薯X病毒RNA末端结构分析
Analysis of terminal structures of RNA from potato virus X.
作者信息
Sonenberg N, Shatkin A J, Ricciardi R P, Rubin M, Goodman R M
出版信息
Nucleic Acids Res. 1978 Jul;5(7):2501-12. doi: 10.1093/nar/5.7.2501.
Abstract
The 5'-end structure of potato virus X RNA was determined following enzymatic methylation in vitro. A single 3H-methyl group was introduced into the 2'-position of the 5'-penultimate residue and the end structure was determined as m7GpppG(m)pAp(Xp)3G. This part of the RNA apparently is involved in binding to ribosomes since it can be partially protected against RNase digestion by wheat germ 40S ribosomes. PVX RNA was not retained by poly(U)-sepharose, indicating that it does not contain a 3'-terminal poly(A) tract.
摘要
通过体外酶促甲基化确定了马铃薯X病毒RNA的5'-末端结构。在5'-倒数第二个残基的2'-位置引入了单个3H-甲基基团，末端结构确定为m7GpppG(m)pAp(Xp)3G。RNA的这一部分显然参与与核糖体的结合，因为它可以被小麦胚芽40S核糖体部分保护免受核糖核酸酶消化。聚尿苷琼脂糖不能保留PVX RNA，表明它不含有3'-末端聚腺苷酸序列。
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本文引用的文献
1
A PROPOSED GENERAL PROCEDURE FOR ISOLATING END-GROUPS OF NUCLEIC ACIDS.一种用于分离核酸末端基团的通用方法建议
Biochemistry. 1963 Jul-Aug;2:703-6. doi: 10.1021/bi00904a014.
2
Sequence studies of satellite tobacco necrosis virus RNA. Isolation and characterization of a 5'-terminal trinucleotide.卫星烟草坏死病毒RNA的序列研究。5'-末端三核苷酸的分离与特性分析。
J Mol Biol. 1968 Nov 28;38(1):59-73. doi: 10.1016/0022-2836(68)90128-9.
3
Valine-specific tRNA-like structure in turnip yellow mosaic virus RNA.芜菁黄花叶病毒RNA中的缬氨酸特异性类tRNA结构
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1345-52. doi: 10.1073/pnas.67.3.1345.
4
Terminal heterogeneity at both ends of the satellite tobacco necrosis virus ribonucleic acid.卫星烟草坏死病毒核糖核酸两端的末端异质性
Biochemistry. 1971 Dec 7;10(25):4748-52. doi: 10.1021/bi00801a022.
5
Absence of polyadenylic acid from reovirus messenger ribonucleic acid.呼肠孤病毒信使核糖核酸中不存在聚腺苷酸。
J Biol Chem. 1973 Dec 10;248(23):7993-8.
6
Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus.豇豆花叶病毒核糖核酸中的聚腺苷酸序列。
Virology. 1973 Nov;56(1):198-206. doi: 10.1016/0042-6822(73)90299-7.
7
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Eur J Biochem. 1972 Dec 4;31(2):246-54. doi: 10.1111/j.1432-1033.1972.tb02527.x.
8
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J Virol. 1972 Nov;10(5):909-19. doi: 10.1128/JVI.10.5.909-919.1972.
9
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Annu Rev Biochem. 1974;43(0):621-42. doi: 10.1146/annurev.bi.43.070174.003201.
10
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Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799-803. doi: 10.1073/pnas.70.6.1799.
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