Watabe T, Miyaji M, Nishimura K
Mycopathologia. 1984 May 30;86(2):113-20. doi: 10.1007/BF00436496.
The histopathology of murine cryptococcosis was observed until the 55th day and particular attention was paid to whether or not cysts, which had been formed in the brain, could change to granulomas. Cryptococcus neoformans RIB-12M was used in this experiment. As experimental animals, five-week-old male BALB/c mice, weighing 20-22 g, were used. An infective inoculum was prepared by adjusting the number of cryptococci to 10(6) or 5 X 10(6)/0.2 ml. Each mouse was inoculated intravenously with 0.2 ml of the cell suspension, and the colony forming unit of the brain and liver, and the histopathological findings in various visceral organs were investigated. 40 X 10(4) colonies grew from 100 mg of the brain tissue of the eighth day. Thereafter, the number increased gradually. It reached 500 X 10(4) on the 20th day. The colony forming unit from the liver reached a peak on the 12th day (250 X 10(4] and thereafter the number decreased gradually. Histopathologically, the brain and liver were severely affected with the fungus. In the brain cysts with cryptococci continued to increase until the end of the experiment. On the other hand, in the liver several purulent foci appeared on the second day. On the eighth day numerous mononuclear cells accumulated at the foci and their lesions changed to granulomatous ones with cryptococci. The number of granulomatous lesions reached a peak on the 16th day in the mice inoculated with 5 X 10(6) cryptococci, and thereafter showed a tendency to decrease gradually.
观察鼠隐球菌病的组织病理学直至第55天，特别关注在脑中形成的囊肿是否会转变为肉芽肿。本实验使用新型隐球菌RIB - 12M。作为实验动物，选用5周龄、体重20 - 22克的雄性BALB/c小鼠。通过将隐球菌数量调整为10(6)或5×10(6)/0.2 ml来制备感染接种物。每只小鼠静脉注射0.2 ml细胞悬液，然后研究脑和肝脏的菌落形成单位以及各个内脏器官的组织病理学发现。在第8天，100毫克脑组织中长出40×10(4)个菌落。此后，数量逐渐增加。在第20天达到500×10(4)。肝脏的菌落形成单位在第12天达到峰值（250×10(4)），此后数量逐渐减少。组织病理学上，脑和肝脏受到真菌的严重影响。在脑中，含隐球菌的囊肿持续增加直至实验结束。另一方面，在肝脏中，第二天出现了几个脓性病灶。在第8天，大量单核细胞在病灶处聚集，其病变转变为含隐球菌的肉芽肿性病变。在接种5×10(6)个隐球菌的小鼠中，肉芽肿性病变的数量在第16天达到峰值，此后呈逐渐下降趋势。
