Bernard A, Lauwerys R, Gengoux P, Mahieu P, Foidart J M, Druet P, Weening J J
Toxicology. 1984 Jun;31(3-4):307-13. doi: 10.1016/0300-483x(84)90111-2.
Sprague-Dawley and Brown-Norway female rats were chronically exposed to cadmium. The metal was administered either in drinking water at a concentration of 20 or 100 ppm for 13 months or intraperitoneally (i.p.) at the dose of 1 mg/kg, 5 times a week for 4 months. Anti-laminin and anti-type IV procollagen antibodies (laminin and type IV procollagen are components of the glomerular basement membranes) were sequentially assessed by radioimmunoassay in serial serum samples. Anti-type IV procollagen antibodies were never detectable whereas anti-laminin antibodies were transiently found in the serum of Sprague-Dawley rats only. In the i.p. group, the antibodies were detected between week 4 and 8, whereas in the oral treatment group, they were detected between month 6 and 8. At the same time, the concentration of cadmium in kidney cortex averaged about 100 ppm in the 20 ppm cadmium group and about 200 ppm in the 2 other treatment groups. The pathogenic significance of these antibodies in chronic cadmium intoxication of Sprague-Dawley rats remains to be assessed since their occurrence was not associated with concomitant immunoglobulin deposits in the kidneys.
将Sprague-Dawley和Brown-Norway雌性大鼠长期暴露于镉环境中。金属通过以下两种方式给予：以20或100 ppm的浓度添加到饮用水中，持续13个月；或者以1 mg/kg的剂量腹腔注射，每周5次，持续4个月。通过放射免疫分析法对系列血清样本依次检测抗层粘连蛋白抗体和抗IV型原胶原抗体（层粘连蛋白和IV型原胶原是肾小球基底膜的组成成分）。从未检测到抗IV型原胶原抗体，而仅在Sprague-Dawley大鼠血清中短暂发现了抗层粘连蛋白抗体。在腹腔注射组中，抗体在第4至8周被检测到，而在口服治疗组中，抗体在第6至8个月被检测到。同时，20 ppm镉组肾皮质中的镉浓度平均约为100 ppm，其他两个治疗组约为200 ppm。这些抗体在Sprague-Dawley大鼠慢性镉中毒中的致病意义仍有待评估，因为它们的出现与肾脏中伴随的免疫球蛋白沉积无关。
