Strel'tsov P G, Filatov M V
Radiobiologiia. 1990 Mar-Apr;30(2):279-82.
A sensitive method for estimating the rate of repair synthesis is proposed. The method is based on the capacity of long-wave UV light to inactivate cells having minor amounts of bromodeoxyuridine (BUdR) incorporated. This treatment is absolutely nontoxic for cells lacking BUdR. The efficacy of the treatment is achieved by sensitizing cells with a fluorescent dye Hoechst 33258. The proposed method has proved to be effective in studying the repair synthesis in HeLa cells after exposure to short-wave UV light and gamma rays.
提出了一种估算修复合成速率的灵敏方法。该方法基于长波紫外光使掺入少量溴脱氧尿苷（BUdR）的细胞失活的能力。这种处理对缺乏BUdR的细胞完全无毒。通过用荧光染料Hoechst 33258使细胞致敏来实现处理的效果。所提出的方法已证明在研究HeLa细胞经短波紫外光和γ射线照射后的修复合成方面是有效的。
