[Sensitivity of the isolated adipose tissue to insulin in patients with insulin-resistant form of diabetes mellitus].

A study was made of the sensitivity of the isolated adipose tissue to insulin in 15 healthy persons, 20-with newly-revealed diabetes mellitus, and in 25-with the insulin-resistant form of diabetes mellitus. Adipose tissue was obtained from the left inquinal region and was incubated with various insulin concentration with the addition of 14C-glucose into the incubation medium, in incubation in buffer, without any insulin addition, of the isolated adipose tissue of patients suffering from insulin-resistant diabetes, 14C-glucose incorporation into the tissue lipids was at the same level as in healthy persons, apparently on account of insulin with prolonged action administered previously. The level of adipose tissue lipids radioactivity in patients suffering from newly-revealed diabetes mellitus and of patients with the insulin-resistant diabetes was the same in incubation with the high insulin concentrations (1000 muU/ml), but significantly lower than in healthy persons. In incubation of the adipose tissue of the insulin-resistant patients in the serum of the same patients, the exogenous insulin administered to the patients and added in vitro failed to express its biological action. A factor preventing the effect of insulin (probably of antibodies of significance in the pathogenesis of insulin resistance) is supposed to be present in the serum of insulin-resistant patients.