[Effect of blood serum of healthy persons and diabetic patients on glucose metabolism in the adipose tissue].

The intensity of lipid synthesis from the C-14-labeled glucose in the isolated subcutaneous fatty tissue of healthy individuals and diabetic patients was investigated during incubation of the tissue in the serum of healthy individuals and of those suffering from diabetes mellitus. It was shown that the serum of healthy individuals and of patients with diabetes mellitus enhanced the lipid synthesis in the adipose tissue of healthy individuals (significantly and equally) in comparison with the incubation in buffer solution. But no lipid synthesis was stimulated under the effect of the serum of healthy persons and patients with diabetes mellitus in the adipose tissue of diabetic patients. The data obtained indicated that antagonists to the insulin action on adipose tissue were absent in the blood of patients with diabetes mellitus. A reduction of the adipose tissue sensitivity of diabetic patients to insulin contained in the serum was due to the tissue defect representing the primary factor in the pathogenesis of the diabetes mellitus responsible for the development of relative insulin deficiency.