Périer C, Baril A, Bayle J J, Janin J, Frey J
Biomed Pharmacother. 1984;38(2):110-4.
In this work, we studied the changes in human skin collagen occurring in diabetes mellitus and liver cirrhosis. The original methodology, based on the determination of the amino acids proline, 4-hydroxyproline, hydroxylysine, glycine and alanine, allowed us to reveal in skin a change in collagen in diabetes mellitus but none in liver cirrhosis. This biochemical evidence was correlated to the histological investigation. Moreover, diabetes mellitus did not involve any changes in hydroxylation of polypeptidic lysine. This latter observation was in accordance with the accumulation of normal collagen regarding amino acid composition only, and the results suggest a preferential accumulation of collagen type III in skin, in diabetes mellitus.
在这项研究中，我们研究了糖尿病和肝硬化患者皮肤中胶原蛋白的变化。基于脯氨酸、4-羟脯氨酸、羟赖氨酸、甘氨酸和丙氨酸等氨基酸测定的原始方法，使我们能够揭示糖尿病患者皮肤中胶原蛋白的变化，而肝硬化患者皮肤中则无变化。这一生物化学证据与组织学研究相关。此外，糖尿病并未涉及多肽赖氨酸羟基化的任何变化。后一观察结果仅与正常胶原蛋白在氨基酸组成方面的积累一致，结果表明糖尿病患者皮肤中优先积累III型胶原蛋白。
