Sundberg M W, Lauff J J, Weiss J S, Dappen G M, Wu T W, Spayd R W, Seligson D
Clin Chem. 1984 Aug;30(8):1314-7.
We used two coated thin films to measure the concentrations of unconjugated, conjugated, and total bilirubin as well as bilirubin covalently bound to albumin ("delta" bilirubin) in more than 400 serum samples. We measured the unconjugated and conjugated species by determining their reflection densities at two wavelengths (400 and 460 nm) on a coating designed for the enhanced spectral measurement of bilirubin but which does not register the delta form. Total bilirubin was measured by use of a diazo-based thin film (Clin Chem 29: 37-41, 1983). We estimated the concentration of delta bilirubin by subtracting the sum of unconjugated and conjugated bilirubin from the concentration of total bilirubin. All measurements agree well with those by comparative methods, as shown by linear regression. Slopes ranged from 0.92 to 1.02, correlation coefficients from 0.935 and 0.998. Linear combinations of these values can also be used to compute other results; e.g., the sum of conjugated and delta bilirubin can be considered to be an estimate of "direct"-reacting bilirubin.
我们使用两种包被薄膜对400多个血清样本中的非结合胆红素、结合胆红素、总胆红素以及与白蛋白共价结合的胆红素（“δ”胆红素）浓度进行了测定。我们通过在一种为增强胆红素光谱测量而设计但不记录δ形式的包被上，于两个波长（400和460 nm）处测定其反射密度，来测量非结合和结合胆红素种类。总胆红素采用基于重氮的薄膜进行测定（《临床化学》29: 37 - 41, 1983）。我们通过从总胆红素浓度中减去非结合胆红素和结合胆红素的总和来估算δ胆红素的浓度。如线性回归所示，所有测量结果与比较方法的测量结果吻合良好。斜率范围为0.92至1.02，相关系数为0.935至0.998。这些值的线性组合也可用于计算其他结果；例如，结合胆红素和δ胆红素的总和可被视为“直接”反应胆红素的估算值。
