Adachi Y, Inufusa H, Yamashita M, Kambe A, Yamazaki K, Sawada Y, Yamamoto T
Second Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Gastroenterol Jpn. 1988 Jun;23(3):268-72. doi: 10.1007/BF02779469.
Serum Bilirubin was fractionated by newly developed reversed phase high performance liquid chromatography (HPLC) into 5 fractions: delta (delta-Bilirubin, B delta), gamma (bilirubin diglucuronide, BDG), beta (Bilirubin monoglucuronide, BMG), beta' ((Z, E,)- and/or (E, Z)-bilirubin IX alpha) and alpha ((Z, Z)-bilirubin IX alpha). Sera of healthy subjects and of patients with unconjugated hyperbilirubinemia showed predominantly alpha fraction with a small amount of beta' fraction. Trace amounts of delta fraction were detected in a few cases. The results of fractionation of serum bilirubin in 159 patients with various hepatobiliary diseases suggested that the ratios B delta/(B delta + BDG + BMG) and BMG/B delta can be useful parameters to follow patients with jaundice, compared with the reported B delta/total bilirubin which did not always reflect the jaundice stage, especially in cases with low serum bilirubin levels.
血清胆红素通过新开发的反相高效液相色谱法（HPLC）分离为5个组分：δ（δ-胆红素，Bδ）、γ（胆红素二葡萄糖醛酸酯，BDG）、β（胆红素单葡萄糖醛酸酯，BMG）、β′（（Z，E）-和/或（E，Z）-胆红素IXα）和α（（Z，Z）-胆红素IXα）。健康受试者和未结合型高胆红素血症患者的血清主要显示为α组分，伴有少量β′组分。少数病例中检测到微量的δ组分。159例各种肝胆疾病患者的血清胆红素分离结果表明，与报道的Bδ/总胆红素相比，Bδ/（Bδ + BDG + BMG）和BMG/Bδ比值可能是跟踪黄疸患者的有用参数，Bδ/总胆红素并不总是能反映黄疸阶段，尤其是在血清胆红素水平较低的情况下。
