Vreman H J, Kwong L K, Stevenson D K
Clin Chem. 1984 Aug;30(8):1382-6.
We examined the sensitive assay for carboxyhemoglobin based on reaction with K3Fe(CN)6 and gas chromatography of the liberated CO. Our improvements included increased baseline stability, shorter analysis time, and simpler standardization. EDTA-containing Vacutainer Tubes (lavender-stoppered) increase the carboxyhemoglobin content of blood stored in them. The carboxyhemoglobin content of blood stored in capillary tubes containing solid heparin and saponin remained stable for two weeks. Using our improved procedures, we measured the carboxyhemoglobin content of blood from adults and neonates collected via venipuncture or heel or fingersticks. We observed no significant difference in carboxyhemoglobin content of blood obtained by venipuncture or heel stick for premature infants, 0.19 +/- 0.04 vs 0.18 +/- 0.03 mL of CO per 100 mL of blood, respectively (mean +/- SD). Nonsmoking adults (n = 19) had CO values (mean +/- SD) of 0.19 +/- 0.03 and 0.17 +/- 0.04 mL per 100 mL of blood, and smoking adults (n = 7) gave CO values of 0.96 +/- 0.49 and 0.91 +/- 0.49 mL/dL, for venipuncture and fingerstick, respectively.
我们基于与K3Fe(CN)6的反应及对释放出的CO进行气相色谱分析，研究了检测碳氧血红蛋白的灵敏方法。我们的改进包括提高基线稳定性、缩短分析时间以及简化标准化流程。含乙二胺四乙酸（EDTA）的真空采血管（紫色帽）会增加其中所储存血液的碳氧血红蛋白含量。储存在含有固体肝素和皂角苷的毛细管中的血液，其碳氧血红蛋白含量在两周内保持稳定。采用我们改进后的方法，我们测量了通过静脉穿刺、足跟或手指采血获取的成人及新生儿血液中的碳氧血红蛋白含量。对于早产儿，我们观察到通过静脉穿刺或足跟采血获得的血液中碳氧血红蛋白含量无显著差异，分别为每100毫升血液中含0.19±0.04毫升和0.18±0.03毫升CO（均值±标准差）。非吸烟成年人（n = 19）每100毫升血液的CO值（均值±标准差），静脉穿刺为0.19±0.03，手指采血为0.17±0.04；吸烟成年人（n = 7）静脉穿刺和手指采血的CO值分别为0.96±0.49和0.91±0.49毫升/分升。
