Winek C L, Prex D M
Forensic Sci Int. 1981 Sep-Oct;18(2):181-7. doi: 10.1016/0379-0738(81)90158-4.
Twenty-one autopsy blood samples were analyzed using spectrophotometric and gas chromatographic procedures after storage for 30 and 150 days. When carboxyhemoglobin was measured spectrophotometrically at the absorbance ratio of 540 nm/555 nm, the observed average percent losses were 8 +/- 9% and 35 +/- 27% after 30 and 150 days of storage, respectively. When measured at the absorbance ratio of 540 nm/579 nm, the average percent losses of carboxyhemoglobin were 7 +/- 8% and 34 +/- 25% after 30 and 150 days, respectively. Wavelength shifts and distorted spectral scans were observed at 150 days. When carbon monoxide was determined by gas chromatographic methods based on combining capacity, the average percent loss was 15 +/- 24% and 37 +/- 36% after 30 days and 150 days, respectively. The average percent loss of calculated CO based on hemoglobin concentration after 30 days was 31 +/- 14% and at 150 days, 40 +/- 24%. The average percent loss of calculated CO based on iron content was 23 +/- 13% and 37 +/- 23% after 30 and 150 days, respectively.
对21份尸检血样在储存30天和150天后，使用分光光度法和气相色谱法进行了分析。当用分光光度法在540 nm/555 nm的吸光度比值下测量碳氧血红蛋白时，储存30天和150天后观察到的平均损失百分比分别为8±9%和35±27%。当在540 nm/579 nm的吸光度比值下测量时，碳氧血红蛋白的平均损失百分比在30天和150天后分别为7±8%和34±25%。在150天时观察到波长偏移和光谱扫描失真。当通过基于结合能力的气相色谱法测定一氧化碳时，30天和150天后的平均损失百分比分别为15±24%和37±36%。基于血红蛋白浓度计算的一氧化碳在30天后的平均损失百分比为31±14%，在150天时为40±24%。基于铁含量计算的一氧化碳在30天和150天后的平均损失百分比分别为23±13%和37±23%。
