Marrie T J, Cooper J H, Costerton J W
Infect Immun. 1984 Aug;45(2):390-8. doi: 10.1128/iai.45.2.390-398.1984.
We used light microscopy and scanning and transmission electron microscopy to study the structure of Candida parapsilosis vegetations on five surgically removed porcine bioprosthetic valves. Scanning electron microscopy revealed a "mossy" amorphous surface. In some areas, C. parapsilosis cells could be seen below the surface layer. In areas in which the vegetation had cracked, myriads of healthy C. parapsilosis cells were seen to be loosely adherent to one another. These cells were covered with a flocculent or fibrillar matrix. A compact intercellular matrix was evident by transmission electron microscopy. Hyphal elements were seen invading valvular tissue. The loose matrix that bound large clumps of C. parapsilosis cells explains why large emboli are frequent complications of Candida endocarditis.
我们使用光学显微镜、扫描电子显微镜和透射电子显微镜来研究五枚手术切除的猪生物瓣膜上近平滑念珠菌赘生物的结构。扫描电子显微镜显示出“苔藓样”无定形表面。在某些区域，可在表层下方看到近平滑念珠菌细胞。在赘生物出现裂缝的区域，可见大量健康的近平滑念珠菌细胞彼此松散附着。这些细胞覆盖着絮状或纤维状基质。透射电子显微镜显示有致密的细胞间基质。可见菌丝成分侵入瓣膜组织。结合大量近平滑念珠菌细胞的松散基质解释了为什么大栓子是念珠菌性心内膜炎的常见并发症。
