Maisch P A, Calderone R A
Infect Immun. 1980 Feb;27(2):650-6. doi: 10.1128/iai.27.2.650-656.1980.
The adherence of Candida albicans to a fibrin-platelet matrix formed in vitro was studied. Platelet-rich plasma obtained from rabbits was incubated with thrombin and CaCl2 to form a clot in tissue culture dishes. Such clots were then infected with 3 x 10(7) C. albicans cells per 0.3 ml prelabeled with [U14C]-glucose, and the percent adherence was measured after 30 min of incubation by counting the radioactivity in saline washes of the clot as well as a streptokinase-streptodornase digest of the corresponding clot. Heat- and formaldehyde-killed cells did not adhere as well as viable cells. Pretreatment of C. albicans with trypsin, chymotrypsin, and pronase reduced adherence to the clots. Normal rabbit serum and anti-Candida antiserum also inhibited adherence 40 and 100%, respectively, Diethylaminoethyl-purified anti-Candida gamma globulin (1:8) completely inhibited adherence, whereas purified normal serum gamma globulin did not. Several Candida spp. and Saccharomyces cerevisiae showed differences in their ability to adhere to clots. C. albicans and C. stellatoidea presented the highest adherence, whereas C. krusei, C. guilliermondii, and S. cerevisiae adhered less readily. Other species were intermediate in their ability to adhere.
研究了白色念珠菌对体外形成的纤维蛋白 - 血小板基质的黏附情况。从兔子身上获取富含血小板的血浆，与凝血酶和氯化钙一起在组织培养皿中孵育以形成凝块。然后用每0.3 ml预先用[U14C] - 葡萄糖标记的3×10(7)个白色念珠菌细胞感染这些凝块，孵育30分钟后，通过计数凝块生理盐水冲洗液以及相应凝块的链激酶 - 链道酶消化液中的放射性来测量黏附百分比。经加热和甲醛处理杀死的细胞不如活细胞黏附得好。用胰蛋白酶、糜蛋白酶和链霉蛋白酶预处理白色念珠菌会降低其对凝块的黏附。正常兔血清和抗念珠菌抗血清也分别抑制黏附40%和100%，二乙氨基乙基纯化的抗念珠菌γ球蛋白（1:8）完全抑制黏附，而纯化的正常血清γ球蛋白则无此作用。几种念珠菌属和酿酒酵母在黏附凝块的能力上存在差异。白色念珠菌和星状念珠菌表现出最高的黏附性，而克鲁斯念珠菌、季也蒙念珠菌和酿酒酵母黏附性较差。其他物种的黏附能力处于中间水平。
