Marrie T J, Costerton J W
J Clin Microbiol. 1985 Dec;22(6):924-33. doi: 10.1128/jcm.22.6.924-933.1985.
Direct examination of material from two cases of persistent (2 and 60 years) osteomyelitis by morphological and culture techniques showed that the pathogens comprised several bacterial species whose cells grew predominantly in discrete exopolysaccharide-enclosed microcolonies made up of a single bacterial morphotype. Bacterial microcolonies were seen between tissue elements in infected connective tissue, and the microcolonies adherent to bone surfaces coalesced to form extensive biofilms that occluded the surfaces of dead bone in sequestrae. Decalcification techniques were required to examine the interior of infected bone, but recognizable remnants were associated with very large amounts of fibrous, ruthenium red-stained material. All bacterial growth in these persistent infections occurred within an intercellular matrix, and some elements of this matrix, which was fibrous in transmission electron microscopy and amorphous in scanning electron microscopy, were associated with the surfaces of bacterial cells in a manner that suggested their production by these organisms. All of the implications of this microcolony mode of bacterial growth in osteomyelitis, and in other chronic bacterial diseases, have yet to be determined.
通过形态学和培养技术对两例持续性（2年和60年）骨髓炎病例的材料进行直接检查发现，病原体包括几种细菌物种，其细胞主要生长在由单一细菌形态型组成的离散的、被胞外多糖包裹的微菌落中。在感染的结缔组织的组织成分之间可见细菌微菌落，并且附着在骨表面的微菌落合并形成广泛的生物膜，这些生物膜封闭了死骨在死骨片中的表面。需要脱钙技术来检查感染骨的内部，但可识别的残留物与大量纤维状、钌红染色物质有关。在这些持续性感染中，所有细菌生长都发生在细胞间基质内，并且该基质的一些成分，在透射电子显微镜下呈纤维状，在扫描电子显微镜下呈无定形，以一种表明由这些生物体产生的方式与细菌细胞表面相关。这种细菌在骨髓炎以及其他慢性细菌疾病中的微菌落生长模式的所有影响尚未确定。
