Naveh A, Potasman I, Bassan H, Ulitzur S
J Appl Bacteriol. 1984 Jun;56(3):457-63. doi: 10.1111/j.1365-2672.1984.tb01374.x.
A new sensitive, rapid and simple bioluminescence assay for antibiotics inhibiting protein synthesis is described. In this assay the ability of the tested antibiotic to inhibit the de novo synthesis of the enzymes participating in the bacterial luminescence system is determined by means of a dark variant of a luminous bacterium that undergoes prompt induction of the luminescence system with certain DNA-intercalating agents. Upon induction, the in vivo luminescence of the dark variant is increased more than 50-fold within 30 min. Antibiotics that block the de novo synthesis of protein limit the development of luminescence at a level that was found to be a function of the antibiotic concentration. The minimum detectable concentration of antibiotics in the bioluminescence test, after 45-60 min of incubation, was 0.1 microgram/ml for streptomycin, gentamicin, kanamycin, lincomycin and chloramphenicol and 0.3 microgram/ml for neomycin, clindamycin and spectinomycin. The new bioluminescence test has been used to assay these antibiotics in serum.
本文描述了一种用于检测抑制蛋白质合成的抗生素的灵敏、快速且简便的生物发光测定法。在该测定法中，通过一种发光细菌的黑暗变体来确定受试抗生素抑制参与细菌发光系统的酶从头合成的能力，这种黑暗变体在某些DNA嵌入剂作用下能迅速诱导发光系统。诱导后，黑暗变体的体内发光在30分钟内增加超过50倍。阻断蛋白质从头合成的抗生素会将发光的发展限制在一个与抗生素浓度有关的水平。孵育45 - 60分钟后，生物发光试验中抗生素的最低可检测浓度，链霉素、庆大霉素、卡那霉素、林可霉素和氯霉素为0.1微克/毫升，新霉素、克林霉素和壮观霉素为0.3微克/毫升。这种新的生物发光试验已用于检测血清中的这些抗生素。
