Barot M S, Bokkenheuser V D
J Clin Microbiol. 1984 Jul;20(1):77-80. doi: 10.1128/jcm.20.1.77-80.1984.
Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU as did solid media but permitted the screening of 50-times larger volumes. Double-strength enrichment medium required 5 to 10 CFU for growth. Omission of lauryl sulfate reduced the sensitivity. Replacement of Butzler antibiotic mixture with Blaser antibiotic mixture increased overgrowth and, therefore, decreased retrieval of C. jejuni.
在所检测的培养基中,添加5%裂解羊血、布茨勒抗生素混合物和0.1%月桂基硫酸盐的硫乙醇酸盐肉汤,是从鸡盲肠内容物和鸡肝脏中分离空肠弯曲菌野生型菌株最敏感的增菌培养基。它与固体培养基一样能检出1个菌落形成单位(CFU),但能筛查的样本量比固体培养基大50倍。双倍强度增菌培养基生长需要5至10个CFU。省略月桂基硫酸盐会降低敏感性。用布拉泽抗生素混合物替代布茨勒抗生素混合物会增加过度生长,因此会减少空肠弯曲菌的检出率。